{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:27:19Z","timestamp":1759332439652,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T00:00:00Z","timestamp":1560729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp; Gen Med"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of <jats:italic>BCR\u2010ABL<\/jats:italic> fusion gene (GenBank accession <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"NC_000022.11\">NC_000022.11<\/jats:ext-link>). In the vast majority of CML patients, the typical subtype of <jats:italic>BCR\u2010ABL<\/jats:italic> transcript are b3a2, b2a2 or both. The aim of this study was to determine the different subtypes of <jats:italic>BCR\u2010ABL<\/jats:italic> transcript and their impact on the demographic and hematological parameters in Iraqi patients with CML.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred patients with chronic phase CML (11 newly diagnosed and 89 imatinib\u2010resistant) were enrolled in this study. Ribonucleic acid (RNA) was extracted from leukocytes, and complementary DNA was created using reverse transcriptase polymerase chain reaction technique. A multiplex polymerase chain reaction with four specific primers was used to determine the <jats:italic>BCR\u2010ABL<\/jats:italic> fusion subtypes in each patient.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Male to female ratio was 1.38:1. Fifty\u2010nine patients expressed b3a2 transcript, whereas 39 of the remaining cases were positive for b2a2 variant. One case expressed b2a3 transcript, while the last case coexpressed the two subtypes of mRNA b3a2\/b2a2. Male and female were significantly associated with b3a2 and b2a2 subtypes, respectively. The b3a2 subtype showed higher total leukocyte count than b2a2 subgroup, while b2a2 variant demonstrated significantly elevated platelet counts compared to those with b3a2 transcript. A significantly higher plateletcrit percentage (PCT%) was found in patients with b2a2 transcript whereas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The testified Iraqi group expressed M\u2010<jats:italic>BCR\u2010ABL<\/jats:italic> type with preponderance of b3a2 over b2a2 subtype. There was a gender\u2010skewed distribution in <jats:italic>BCR\u2010ABL<\/jats:italic> transcript types with b3a2 transcript more prevalent in males. The type of <jats:italic>BCR\u2010ABL<\/jats:italic> transcript is reflected by different leukocyte and platelet counts at diagnosis, which might represent a distinct phenotype and disease biology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.809","type":"journal-article","created":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T15:34:05Z","timestamp":1560785645000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Association of <i>BCR\/ABL<\/i> transcript variants with different blood parameters and demographic features in Iraqi chronic myeloid leukemia patients"],"prefix":"10.1002","volume":"7","author":[{"given":"Mahmood S.","family":"Khazaal","sequence":"first","affiliation":[{"name":"Department of Physiology, College of Medicine Al\u2010Nahrain University Baghdad Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4994-3694","authenticated-orcid":false,"given":"Farqad B.","family":"Hamdan","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine Al\u2010Nahrain University Baghdad Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qasim S.","family":"Al\u2010Mayah","sequence":"additional","affiliation":[{"name":"Medical Research Unit, College of Medicine Al\u2010Nahrain University Baghdad Iraq"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2008.01170.x"},{"issue":"2","key":"e_1_2_8_3_1","first-page":"444","article-title":"Correlation of p210 BCR\u2010ABL transcript variants with clinical, parameters and disease outcome in 45 chronic myeloid leukemia 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