{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T14:21:32Z","timestamp":1772720492304,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,7,21]],"date-time":"2019-07-21T00:00:00Z","timestamp":1563667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molec Gen &amp; Gen Med"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>It has been proposed that lncRNAs, widely transcribed from genomes, play pivotal regulatory roles in a variety of biological processes, but their function in regulating spermatogenesis in human males is rarely reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>QRT\u2010PCR was adopted to detect <jats:italic>HOTTIP<\/jats:italic> expression level in testicular tissues from hypospermatogenesis (Hypo) patients or controls. The proliferation levels of NT2 and 293T were measured <jats:italic>via<\/jats:italic> CCK\u20108 and EdU detection. Meanwhile, luciferase reporter gene assay and bioinformatics analysis were carried out to identify a target of <jats:italic>HOTTIP<\/jats:italic>. Additionally, the underlying mechanism of <jats:italic>HOTTIP<\/jats:italic>\u2019s function was investigated using western blotting and RIP analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The research results manifested that the expression of <jats:italic>HOTTIP<\/jats:italic> in testicular tissues from Hypo patients was prominently reduced in comparison with that in control testicular tissues. Interestingly, it was noted that <jats:italic>HOTTIP<\/jats:italic> exhibited a high expression in testicular embryonal carcinoma cell line NT2 compared with that in normal control cell line 293T. It was denoted in cell function evaluation that cell proliferation was impeded by downregulated <jats:italic>HOTTIP<\/jats:italic> but evidently stimulated by overexpressed <jats:italic>HOTTIP<\/jats:italic>. Moreover, <jats:italic>HOTTIP<\/jats:italic> was capable of positively modulating <jats:italic>HOXA13<\/jats:italic> expression <jats:italic>via<\/jats:italic> the competitive binding to miR\u2010128\u20103p.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Therefore, <jats:italic>HOTTIP<\/jats:italic> acting as ceRNAs to promote testicular embryonal carcinoma cell proliferation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mgg3.870","type":"journal-article","created":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T00:39:46Z","timestamp":1563755986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Long noncoding RNA HOTTIP is associated with male infertility and promotes testicular embryonal carcinoma cell proliferation"],"prefix":"10.1002","volume":"7","author":[{"given":"Yang","family":"Su","sequence":"first","affiliation":[{"name":"Department of Surgery The Affiliated Huaian No. 1 People\u2019s Hospital of Nanjing Medical University Huaian Jiangsu China"},{"name":"State Key Laboratory of Reproductive Medicine, Center for Global Health, Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling\u2010Ling","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine, Center for Global Health, Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine, Center for Global Health, Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5314-4700","authenticated-orcid":false,"given":"Liang\u2010Yu","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Andrology Jiangsu Provincial Hospital of Traditional Chinese Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,21]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","first-page":"2","article-title":"Male infertility","volume":"1","author":"Chalyi M. 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