{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:10:05Z","timestamp":1788869405565,"version":"build-2803163510"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,10,8]],"date-time":"2003-10-08T00:00:00Z","timestamp":1065571200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fundo de Aux\u00edlio aos Docentes e Alunos"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Our objective was to evaluate intestinal ischemia\u2010reperfusion injury in growing rats, modulated by hypothermia (I\/RH) and N\u2010acetylcysteine (NAC). We used 30 EPM\u20101 Wistar male rats, aged around 35 days, weighing 90 g. Rats were randomized into 5 groups with 6 animals in each: I\/RH group, intestinal ischemia under hypothermia for 40 min and reperfusion for 30 min; I\/RH\u2010NAC group, same procedure but adding NAC (150 mg\u00b7kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), previously with ischemia; S\u2010H group, topic hypothermia for 40 min, and observation for 30 min; I\/R H\u2010Ve group; and S\u2010NAC group, NAC administration and observation for 70 min. All animals were heparinized and anesthetized with ketamine (60 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) and xylazine (10 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) intramuscularly. Surgical procedures were done under microsurgical technique (augmentation, 10\u00d7). After laparotomy, the superior mesenteric artery was dissected and clamped to promote ischemia. Topic hypothermia was obtained by using plastic bags at 4\u00b0C, changed every 10 min. Rats were sacrificed by exsanguination, and blood samples were utilized to measure Dlactate. Intestinal fragments were removed for morphological study. Statistical analysis was done with nonparametric tests (\n                    <jats:italic>P<\/jats:italic>\n                    \u2264 0.05). Concerning to D\n                    <jats:sup>\u2010<\/jats:sup>\n                    lactate, the data showed biochemical tissue injury, with hypothermia only (S\u2010H = 27 mg\/dl), and this became more important when intestinal ischemia and reperfusion were associated to hypothermia (I\/RH = 36 mg\/dl). NAC decreased ischemia\u2010reperfusion injury (I\/RH\u2010NAC = 19 mg\/dl). Morphologic tissue injuries, evaluated by hematoxylin\u2010eosin staining, showed grades 4 and 5 for the I\/RH and I\/RH\u2010Ve groups, respectively, in contrast with other groups (I\/RH\u2010NAC = 2, S\u2010H = 1, and S\u2010NAC = 1). Based on our data, we conclude that intestinal ischemia reperfusion injury occurred morphologically as well as functionally, even under hypothermia. However, NAC showed a protective effect on the small bowel from ischemia\u2010reperfusion injury.\n                  <\/jats:p>\n                  <jats:p>\n                    Para avaliar a les\u00e3o de isquemia e reperfus\u00e3o em ratos em crescimento, modulada com a hipotermia (I\/RH) e a N\u2010acetilciste\u00edna (NAC), foram usados 30 ratos machos, EPM\u20101 Wistar, com idade de 35 dias, pesando 90 g. Os ratos foram randomizados em 5 grupos com 6 animais cada: grupo I\/RH, isquemia intestinal + hipotermia por 40 min e reperfus\u00e3o por 30 min; grupo I\/RH\u2010NAC, adicionada \u00e0 NAC (150 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), previamente \u00e0 isquemia; grupo simulado\u2010H, hipotermia t\u00f3pica por 40 min e observa\u00e7\u00e3o durante 30 min; grupo I\/RH\u2010ve\u00edculo; e grupo simulado\u2010NAC, administra\u00e7\u00e3o de NAC e observa\u00e7\u00e3o por 70 min. Ap\u00f3s hepariniza\u00e7\u00e3o, os ratos foram anestesiados com cetamina (60 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) e xilazina (10 mg kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), intramuscular. Os procedimentos operat\u00f3rios foram realizados com t\u00e9cnica microcir\u00fargica (aumento, 10\u00d7). Ap\u00f3s laparotomia, a art\u00e9ria mesent\u00e9rica superior foi dissecada e clampeada para promover isquemia. A hipotermia t\u00f3pica foi obtida por meio de bolsas pl\u00e1sticas a 4\u00b0C, mudadas a cada 10 min. Os ratos foram sacrificados por exsang\u00fcina\u00e7\u00e3o e amostras de sangue foram utilizadas para medir o D\n                    <jats:sup>\u2010<\/jats:sup>\n                    lactato. Fragmentos intestinais foram removidos para o estudo morfol\u00f3gico. A an\u00e1lise estat\u00edstica foi feita com teste de Kruskal\u2010Wallis (\n                    <jats:italic>P<\/jats:italic>\n                    \u2264 0.05). Com rela\u00e7\u00e3o ao D\n                    <jats:sup>\u2010<\/jats:sup>\n                    lactato, os dados mostraram les\u00e3o bioqu\u00edmica, com a hipotermia isoladamente (S\u2010H = 27 mg\/dl) que se tornou mais acentuada quando a isquemia e reperfus\u00e3o intestinal foram associadas (I\/RH = 36 mg\/dl). A NAC diminuiu a les\u00e3o de isquemia e reperfus\u00e3o intestinal (I\/RH\u2010NAC = 19 mg\/dl). As les\u00f5es morfol\u00f3gicas, avaliadas na colora\u00e7\u00e3o de HE, mostraram graus 4 e 5 para os grupos I\/R H e I\/R H\u2010Ve, respectivamente, em contraste com os outros grupos (I\/RH\u2010NAC = 2, S\u2010H = 1, e S\u2010NAC = 1). Os achados permitiram concluir que a les\u00e3o de isquemia e reperfus\u00e3o intestinal ocorreu tanto morfologicamente como funcionalmente, mesmo com a associa\u00e7\u00e3o da hipotermia, que isoladamente causou les\u00e3o bioqu\u00edmica. Entretanto, a NAC mostrou um efeito protetor do intestino na les\u00e3o de isquemia e reperfus\u00e3o. \u00a9 2003 Wiley\u2010Liss, Inc. MICROSURGERY 23:517\u2013521 2003\n                  <\/jats:p>","DOI":"10.1002\/micr.10163","type":"journal-article","created":{"date-parts":[[2003,10,10]],"date-time":"2003-10-10T19:02:12Z","timestamp":1065812532000},"page":"517-521","source":"Crossref","is-referenced-by-count":14,"title":["Intestinal ischemia and reperfusion injury in growing rats: Hypothermia and N\u2010acetylcysteine modulation"],"prefix":"10.1002","volume":"23","author":[{"given":"Edna F.S.","family":"Montero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos S.","family":"Abrah\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia K.","family":"Koike","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f4nica C.B.","family":"Manna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E.B.","family":"Ramalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,10,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199205000-00001"},{"key":"e_1_2_1_3_2","first-page":"2595","article-title":"Mechanism and protection from ischemic intestinal injury","volume":"28","author":"Banda A","year":"1996","journal-title":"Transplant Proc"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549-8719.1999.tb00099.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(98)01397-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198804000-00001"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002689900525"},{"key":"e_1_2_1_8_2","first-page":"717","article-title":"Simple in situ hypothermia reduced ischaemic injury to human liver during hepatectomy","volume":"162","author":"Kim YI","year":"1996","journal-title":"Eur J Surg"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1994.01420300062009"},{"key":"e_1_2_1_10_2","first-page":"2402","article-title":"Oxygen\u2010derived radicals: a link between reperfusion injury and inflammation","volume":"46","author":"McCord JM","year":"1987","journal-title":"Fed Proc"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510280201"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(00)01214-8"},{"key":"e_1_2_1_13_2","first-page":"574","article-title":"The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion","volume":"107","author":"Park PO","year":"1990","journal-title":"Surgery"},{"key":"e_1_2_1_14_2","first-page":"224","article-title":"Efeito da hipotermia hep\u00e1tica na les\u00e3o de isquemia e reperfus\u00e3o em ratos","volume":"30","author":"Quireze C","year":"1999","journal-title":"Hepatology"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2000.6062"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1970.01340280030009"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840220225"}],"container-title":["Microsurgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmicr.10163","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/micr.10163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T19:52:10Z","timestamp":1700250730000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/micr.10163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,10]]}},"alternative-id":["10.1002\/micr.10163"],"URL":"https:\/\/doi.org\/10.1002\/micr.10163","archive":["Portico"],"relation":{},"ISSN":["0738-1085","1098-2752"],"issn-type":[{"value":"0738-1085","type":"print"},{"value":"1098-2752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,10]]}}}