{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T17:40:10Z","timestamp":1700415610878},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,7,31]],"date-time":"2001-07-31T00:00:00Z","timestamp":996537600000},"content-version":"vor","delay-in-days":211,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this study was to optimize the methods of retrograde labeling of sensory neurons in demonstrating the continuity of post\u2010ganglionic primary sensory axons. This was accomplished by comparing four different methods of horseradish peroxidase (HRP) application into the lower thoracic spinal cord of adult rats (level T11). HRP application with a piece of Gelfoam via a dorsal myelotomy (group 1, n = 8), stereotactic injections with a 0.72\u2010mm tip diameter needle (group 2, n = 8), with a 0.24\u2010mm tip needle (group 3, n = 8), and with a 0.08\u2010mm tip glass micropipette (group 4, n = 5). Histological examination of the application site showed that the extent of spinal cord injury was directly proportional to the diameter of the needle tip. The mean number of dorsal root ganglia (DRG) sensory neurons retrogradely stained by HRP differed among the four experimental groups: 77 \u00b1 45 (SEM) per DRG in group 1, 106 \u00b1 24 in group 2, 652 \u00b1 90 in group 3, and 238 \u00b1 60 in group 4. A significant difference was found between group 3 and the other ones (<jats:italic>P<\/jats:italic> &lt; 0.05). Intraspinal injection of HRP with a fine needle (0.24\u2010mm tip diameter) using a stereotactic approach can achieve effective and reliable retrograde labeling of primary sensory neurons. This reproducible method may be useful in studies dealing with regeneration of post\u2010ganglionic primary sensory axons. \u00a9 2001 Wiley\u2010Liss, Inc. Microsurgery 21:214\u2013220 2001<\/jats:p>","DOI":"10.1002\/micr.1041","type":"journal-article","created":{"date-parts":[[2002,9,11]],"date-time":"2002-09-11T01:16:37Z","timestamp":1031706997000},"page":"214-220","source":"Crossref","is-referenced-by-count":3,"title":["Horseradish peroxidase retrograde labeling of primary sensory neurons: A comparison of four intraspinal applicaton methods"],"prefix":"10.1002","volume":"21","author":[{"given":"Phong","family":"Dam\u2010Hieu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Song","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne\u2010Sophie","family":"Bonnard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Tadie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,7,31]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)00848-7"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/26.2.24068"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)90328-3"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(95)90027-6"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90563-7"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/309791a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5274.510"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19960826)372:3<435::AID-CNE7>3.0.CO;2-3"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1997.6693"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1998.00071.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1986.tb08024.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19980315)51:6<723::AID-JNR6>3.0.CO;2-D"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19990601)56:5<506::AID-JNR6>3.0.CO;2-I"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902760308"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(05)80011-1"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903040211"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01420053"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902200105"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902500203"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/sc.1986.55"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(76)90174-8"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.1997.19010051.x"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)01314-6"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/35002084"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90005-1"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(97)02227-9"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)90882-9"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7268404"},{"key":"e_1_2_1_30_2","series-title":"The rat nervous system","first-page":"129","volume-title":"the rat nervous hindbrain and spinal cord","author":"Tracey DJ","year":"1985"}],"container-title":["Microsurgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmicr.1041","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/micr.1041","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T17:04:28Z","timestamp":1700413468000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/micr.1041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":29,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1002\/micr.1041"],"URL":"https:\/\/doi.org\/10.1002\/micr.1041","archive":["Portico"],"relation":{},"ISSN":["0738-1085","1098-2752"],"issn-type":[{"value":"0738-1085","type":"print"},{"value":"1098-2752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,1]]}}}