{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T16:39:33Z","timestamp":1777135173066,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,6,27]],"date-time":"2007-06-27T00:00:00Z","timestamp":1182902400000},"content-version":"vor","delay-in-days":177,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Myostatin, a member of the transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) superfamily, has been identified as a negative regulator of skeletal muscle mass. To provide more data on the role of myostatin in denervation\u2010induced muscle atrophy, we examined the time\u2010dependent changes in myostatin mRNA and protein as well as Smad2 and phospho\u2010Smad2 protein levels in the denervated gastrocnemius muscle of mice after sciatic neurectomy, using quantitative real\u2010time RT\u2010PCR and Western blotting, respectively. We conducted morphometric analyses to measure the wet weight ratio of the denervated muscle (the operated side\/contralateral nonoperated side) and the cross\u2010sectional area of muscle fibers, and observed the morphology of denervated muscle. The experimental results showed that in the early stage of denervation, the levels of myostatin mRNA and protein in the denervated gastrocnemius muscle increased instantly, reaching a peak at day 3 and day 7 after sciatic neurectomy, respectively, when compared with the normal values. In addition, the phospho\u2010Smad2 protein was observed to have a similar expression profile to that of the myostatin mRNA. The present study perhaps opens a new window into myostatin modulation in muscle atrophy due to denervation. \u00a9 2007 Wiley\u2010Liss, Inc. Microsurgery, 2007.<\/jats:p>","DOI":"10.1002\/micr.20392","type":"journal-article","created":{"date-parts":[[2007,6,27]],"date-time":"2007-06-27T18:24:21Z","timestamp":1182968661000},"page":"487-493","source":"Crossref","is-referenced-by-count":30,"title":["Time\u2010dependent expression of myostatin RNA transcript and protein in gastrocnemius muscle of mice after sciatic nerve resection"],"prefix":"10.1002","volume":"27","author":[{"given":"Chenxin","family":"Shao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,6,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00579.2003"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/387083a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0997-71"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.23.12457"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.7.9.910"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10306"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(00)00044-6"},{"issue":"1","key":"e_1_2_1_9_2","first-page":"8","article-title":"Myostatin expression in age and denervation\u2010induced skeletal muscle atrophy","volume":"3","author":"Baumann AP","year":"2003","journal-title":"J Musculoskelet Neuronal Interact"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.14.1.103"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.1086"},{"issue":"2","key":"e_1_2_1_12_2","first-page":"11","article-title":"Plasma myostatin\u2010immunoreactive protein is increased after prolonged bed rest with low\u2010dose T3 administration","volume":"6","author":"Zachwieja JJ","year":"1999","journal-title":"J Gravit Physiol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00487.2002"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.25.14938"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069525"},{"issue":"2","key":"e_1_2_1_16_2","first-page":"R601","article-title":"Skeletal muscle myostatin mRNA expression is fiber\u2010type specific and increases during hindlimb unloading","volume":"277","author":"Carlson CJ","year":"1999","journal-title":"Am J Physiol"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-005-9050-5"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004356200"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-1228fje"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.14.6.627"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000099373.33259.B2"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80128-2"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81817-6"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(97)00384-4"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.20.7230-7242.2003"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2004.03.007"}],"container-title":["Microsurgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmicr.20392","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/micr.20392","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T21:07:02Z","timestamp":1699996022000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/micr.20392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/micr.20392"],"URL":"https:\/\/doi.org\/10.1002\/micr.20392","archive":["Portico"],"relation":{},"ISSN":["0738-1085","1098-2752"],"issn-type":[{"value":"0738-1085","type":"print"},{"value":"1098-2752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]}}}