{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:46:27Z","timestamp":1792741587613,"version":"4.5.0"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,7,11]],"date-time":"2008-07-11T00:00:00Z","timestamp":1215734400000},"content-version":"vor","delay-in-days":192,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The goal of the present study was to assess whether in vitro\u2010differentiated N1E\u2010115 cells supported by a collagen membrane would enhance rat sciatic nerve regeneration after a crush injury. To set up an appropriate experimental model for investigating the effects of neural cell transplantation, we have recently described the sequence of functional and morphologic changes occurring after a standardized sciatic nerve crush injury with a nonserrated clamp. Functional recovery was evaluated using the sciatic functional index, the static sciatic index, the extensor postural thrust, the withdrawal reflex latency, and ankle kinematics. In addition, histomorphometric analysis was carried out on regenerated nerve fibers by means of the 2D\u2010disector method. Based on the results of the EPT and of some of the ankle locomotor kinematic parameters analyzed, the hypothesis that N1E\u2010115 cells may enhance nerve regeneration is partially supported although histomorphometry disclosed no significant difference in nerve fiber regeneration between the different experimental groups. Therefore, results suggest that enrichment of equine type III collagen membrane with the N1E\u2010115 cellular system in the rat sciatic nerve crush model may support recovery, at least in terms of motor function. The discrepancy between functional and morphological results also suggests that the combined use of functional and morphological analysis should be recommended for an overall assessment of recovery in nerve regeneration studies. \u00a9 2008 Wiley\u2010Liss, Inc. Microsurgery, 2008.<\/jats:p>","DOI":"10.1002\/micr.20524","type":"journal-article","created":{"date-parts":[[2008,7,11]],"date-time":"2008-07-11T16:59:39Z","timestamp":1215795579000},"page":"458-470","source":"Crossref","is-referenced-by-count":23,"title":["Neural cell transplantation effects on sciatic nerve regeneration after a standardized crush injury in the rat"],"prefix":"10.1002","volume":"28","author":[{"given":"A. L.","family":"Lu\u00eds","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Geuna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Amado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"Sim\u00f5es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Fregnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. P.","family":"Veloso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. A. S.","family":"Armada\u2010Da\u2010Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S. P.","family":"Varej\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C.","family":"Maur\u00edcio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198503000-00014"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ddmod.2004.09.008"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2004.21.1652"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2007.02.017"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/089771504772695986"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1529-8027.2003.03020.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.5.011303.120731"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2006.tb00401.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.30.051606.094337"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)91014-X"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-07-02012.1986"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/360753a0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.1.258"},{"key":"e_1_2_1_15_2","first-page":"323","article-title":"Intracellular Ca2+ concentration in the N1E\u2010115 neuronal cell line and its use for peripheric nerve regeneration","volume":"18","author":"Rodrigues JM","year":"2005","journal-title":"Acta Med Port"},{"key":"e_1_2_1_16_2","first-page":"455","article-title":"Determination of the intracellular Ca2+ concentration in the N1E\u2010115 neuronal cell line in perspective of its use for peripheric nerve regeneration","volume":"15","author":"Rodrigues JM","year":"2005","journal-title":"Biomed Mater Eng"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.2.462"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/S0026-895X(25)09579-3","article-title":"Up\u2010regulation of angiotensin II receptors by in vitro differentiation of murine N1E\u2010115 neuroblastoma cells","volume":"38","author":"Reagan LP","year":"1990","journal-title":"Mol Pharmacol"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)91714-2"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.1994.10718412"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.1991.tb01148.x"},{"key":"e_1_2_1_22_2","article-title":"Use of PLGA 90:10 scaffolds enriched with in vitro\u2010differentiated neural cells for repairing rat sciatic nerve defects","author":"Lu\u00eds AL","journal-title":"Tissue Eng"},{"key":"e_1_2_1_23_2","first-page":"632","article-title":"Phenytoin action on the excitable membrane of mouse neuroblastoma","volume":"211","author":"Tutter JB","year":"1979","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-17755"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(91)90008-L"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(91)90052-2"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20317"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199504000-00026"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7600"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199308000-00020"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199704000-00026"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00388-2"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1179\/016164104225013833"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198901000-00024"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(00)00281-8"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(99)00174-0"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(01)00378-8"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(79)90389-8"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(86)90105-8"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10242"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4886(03)00208-5"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10374"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0940-9602(00)80117-X"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004290100188"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/micr.10199"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20167"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050191"},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970728)384:2<312::AID-CNE10>3.0.CO;2-K"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2006.05.002"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-974649"},{"key":"e_1_2_1_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880141113"},{"key":"e_1_2_1_52_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-198802000-00020"},{"key":"e_1_2_1_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9290(00)00023-3"},{"key":"e_1_2_1_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2003.09.017"}],"container-title":["Microsurgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmicr.20524","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/micr.20524","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:34:15Z","timestamp":1738283655000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/micr.20524"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":53,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1002\/micr.20524"],"URL":"https:\/\/doi.org\/10.1002\/micr.20524","archive":["Portico"],"relation":{},"ISSN":["0738-1085","1098-2752"],"issn-type":[{"value":"0738-1085","type":"print"},{"value":"1098-2752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1]]}}}