{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T22:49:13Z","timestamp":1790549353554,"version":"4.1.0"},"reference-count":9,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T00:00:00Z","timestamp":1361145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Medical leech therapy (MLT) with <jats:italic>Hirudo medicinalis<\/jats:italic> is well established as a treatment for venous congestion of tissue flaps, grafts, and replants. Unfortunately, this treatment is associated with surgical site infections with bacterial species, most commonly <jats:italic>Aeromonas hydrophila<\/jats:italic>, which is an obligate symbiot of <jats:italic>H. medicinalis<\/jats:italic>. For this reason, prophylactic antibiotics are recommended in the setting of MLT. After culturing <jats:italic>Aeromonas<\/jats:italic><jats:italic>hydrophila<\/jats:italic> resistant to ciprofloxacin from a tissue specimen from a patient with a failed replant of three digits post\u2010MLT, we performed environmental surveillance cultures and antibiotic susceptibility testing on water collected from leech tanks. This surveillance was performed twice weekly for 2.5 months. Fourteen surveillance cultures demonstrated 21 isolates of <jats:italic>Aeromonas<\/jats:italic> species, 71.4% of which were ciprofloxacin susceptible. All isolates were sulfamethoxazole\u2010trimethoprim (SXT) susceptible. The prophylactic antibiotic regimen of choice for leech therapy at our institution is SXT, with culture of tank water to refine antimicrobial choice if necessary. This study demonstrates the importance of regular surveillance to detect resistant <jats:italic>Aeromonas<\/jats:italic> species in medical leeches; however optimal practice has not been established. \u00a9 2013 Wiley Periodicals, Inc. Microsurgery, 2013.<\/jats:p>","DOI":"10.1002\/micr.22087","type":"journal-article","created":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T22:38:42Z","timestamp":1361227122000},"page":"301-304","source":"Crossref","is-referenced-by-count":23,"title":["The role of leech water sampling in choice of prophylactic antibiotics in medical leech therapy"],"prefix":"10.1002","volume":"33","author":[{"given":"Amanda","family":"Wilmer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Slater","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Yip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Carr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Grant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20971"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20860"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1608\/FRJ-4.1.417"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20666"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/micr.21960"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/archoto.2010.257"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.12.012"},{"key":"e_1_2_6_9_2","volume-title":"Methods of Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline","author":"Clinical and Laboratory Standards Institute","year":"2010"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20827"}],"container-title":["Microsurgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmicr.22087","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/micr.22087","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T15:18:03Z","timestamp":1700666283000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/micr.22087"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,18]]},"references-count":9,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["10.1002\/micr.22087"],"URL":"https:\/\/doi.org\/10.1002\/micr.22087","archive":["Portico"],"relation":{},"ISSN":["0738-1085","1098-2752"],"issn-type":[{"value":"0738-1085","type":"print"},{"value":"1098-2752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,18]]}}}