{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T20:14:18Z","timestamp":1790367258935,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,3,14]],"date-time":"2015-03-14T00:00:00Z","timestamp":1426291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["23592163"],"award-info":[{"award-number":["23592163"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>Previously, we showed that undifferentiated bone marrow stromal cell (uBMSC) implantation and vessel insertion into a nerve conduit facilitated peripheral nerve regeneration in a rodent model. In this study, we investigated the efficacy of the uBMSC\u2010laden vessel\u2010containing conduit in repair of segmental nerve defects, using a canine model. Eight beagle dogs were used in this study. Thirty\u2010millimeter ulnar nerve defects were repaired with the conduits (right forelimbs,<jats:italic>n<\/jats:italic>\u2009=\u20098) or autografts (left forelimbs,<jats:italic>n<\/jats:italic>\u2009=\u20097). In the conduit group, the ulnar artery was inserted into the l\u2010lactide\/\u03b5\u2010caprolactone tube, which was filled with autologous uBMSCs obtained from the ilium. In the autograft group, the reversed nerve segments were sutured in situ. At 8 weeks, one dog with only nerve repair with the conduit was sacrificed and the regenerated nerve in the conduit underwent immunohistochemistry for investigation of the differentiation capability of the implanted uBMSCs. In the remaining seven dogs, the repaired nerves underwent electrophysiological examination at 12 and 24 weeks and morphometric measurements at 24 weeks. The wet weight of hypothenar muscles was measured at 24 weeks. At 8 weeks, almost 35% of the implanted uBMSCs expressed glial markers. At 12 weeks, amplitude (0.4\u2009\u00b1\u20090.4mV) and conduction velocity (18.9\u2009\u00b1\u200914.3m\/s) were significantly lower in the conduit group than in the autograft group (3.2\u2009\u00b1\u20092.5 mV, 34.9\u2009\u00b1\u200912.1 m\/s,<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Although the nerve regeneration in the conduit group was inferior when compared with the autograft group at 24 weeks, there were no significant differences between both groups, regarding amplitude (10.9\u2009\u00b1\u20097.3 vs. 25.3\u2009\u00b1\u200920.1 mV;<jats:italic>P<\/jats:italic>\u2009=\u20090.11), conduction velocity (23.5\u2009\u00b1\u20098.7 vs 31.6\u2009\u00b1\u200920.0m\/s;<jats:italic>P<\/jats:italic>\u2009=\u20090.35), myelinated axon number (7032\u2009\u00b1\u20094188 vs 7165\u2009\u00b1\u20091814;<jats:italic>P<\/jats:italic>\u2009=\u20090.94), diameter (1.73\u2009\u00b1\u20090.31 vs 2.09\u2009\u00b1\u20090.39\u03bcm;<jats:italic>P<\/jats:italic>\u2009=\u20090.09), or muscle weight (1.02\u2009\u00b1\u20090.40 vs 1.19\u2009\u00b1\u20090.26g;<jats:italic>P<\/jats:italic>\u2009=\u20090.36). In conclusion, this study showed that vessel\u2010containing tubes with uBMSC implantation may be an option for treatment of peripheral nerve injuries. However, further investigations are needed. \u00a9 2015 Wiley Periodicals, Inc. Microsurgery 36:316\u2013324, 2016.<\/jats:p>","DOI":"10.1002\/micr.22391","type":"journal-article","created":{"date-parts":[[2015,3,14]],"date-time":"2015-03-14T05:39:05Z","timestamp":1426311545000},"page":"316-324","source":"Crossref","is-referenced-by-count":20,"title":["Bridging a 30 mm defect in the canine ulnar nerve using vessel\u2010containing conduits with implantation of bone marrow stromal cells"],"prefix":"10.1002","volume":"36","author":[{"given":"Yukitoshi","family":"Kaizawa","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Graduate School of Medicine, Kyoto University Kyoto Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Kakinoki","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Faculty of Medicine, Kindai University Higashiosaka Osaka Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Ikeguchi","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Graduate School of Medicine, Kyoto University Kyoto Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souichi","family":"Ohta","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Graduate School of Medicine, Kyoto University Kyoto Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Graduate School of Medicine, Kyoto University Kyoto Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Graduate School of Medicine, Kyoto University Kyoto Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Graduate School of Medicine, Kyoto University Kyoto Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20127"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.22105"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1179\/016164102101200564"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1179\/016164104225013897"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20184"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2006.12.004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3727\/000000007783465226"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2007.09.098"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20818"},{"key":"e_1_2_6_11_1","first-page":"3779","article-title":"Use of tissue\u2010engineered nerve grafts consisting of a chitosan\/poly(lactic\u2010co\u2010glycolic acid)\u2010based scaffold included with bone marrow mesenchymal cells for bridging 50\u2010mm dog sciatic nerve gaps. 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