{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:29:58Z","timestamp":1746145798810,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T00:00:00Z","timestamp":1437350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"The Japanese Ministry of Education, Science, Sports, and Culture","doi-asserted-by":"crossref","award":["26861499"],"award-info":[{"award-number":["26861499"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Kanae Foundation for the Promotion of Medical Science and The Uehara Memorial Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>One of the major problems with nerve grafts is that the survival of a graft segment, including endoneurial Schwann cells (SCs), is uncertain. We investigated whether the survival of nerve grafts is improved when adipose\u2010derived stem cells (ASCs) are incorporated into the grafts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To examine the cell\u2010protective effects of ASCs on SCs <jats:italic>in vitro<\/jats:italic>, we used an indirect coculture system<jats:italic>. In vivo<\/jats:italic> effects of the incorporation of ASCs into grafts were examined using a graft model in the rat common peroneal nerve. Grafts were entubulated to isolate them from the surrounding tissues, mimicking the clinical conditions of a poorly vascularized recipient bed. Thirty\u2010six Lewis rats were divided into three groups, i.e., nerve graft only, entubulated nerve graft, and entubulated nerve graft\u2009+\u2009ASC transplantation. In each group, four rats and eight rats were used for short\u2010term (10 days) and long\u2010term (12 weeks) follow\u2010up study, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 24 hours of serum deprivation, the numbers of 7\u2010aminoactinomycin D, and TUNEL\u2010positive SCs significantly decreased when indirectly cocultured with ASCs (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). When ASCs were transplanted to the epineurial layer of the grafts, the number of endoneurial TUNEL\u2010positive cells decreased significantly, as compared with grafts without ASCs, at 10 days postoperatively (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Postoperative walking track analysis showed that the ASC\u2010transplanted grafts showed significantly faster function recovery, as compared with grafts without ASCs (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that nerve autografts\u2009+\u2009ASC therapy could offer a new approach to obtaining optimal outcomes after peripheral nerve injury. \u00a9 2015 Wiley Periodicals, Inc. Microsurgery 35:474\u2013480, 2015.<\/jats:p><\/jats:sec>","DOI":"10.1002\/micr.22451","type":"journal-article","created":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T06:25:37Z","timestamp":1437373537000},"page":"474-480","source":"Crossref","is-referenced-by-count":7,"title":["Adipose\u2010derived stem cells protect against endoneurial cell death: Cell therapy for nerve autografts"],"prefix":"10.1002","volume":"35","author":[{"given":"Koichi","family":"Tomita","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Graduate School of Medicine, Osaka University Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akimitsu","family":"Nishibayashi","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Graduate School of Medicine, Osaka University Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Yano","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Graduate School of Medicine, Osaka University Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ko","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Graduate School of Medicine, Osaka University Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1966.01330020127022"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1966.01330030063014"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0030-5898(20)31389-4","article-title":"The anatomic foundation of peripheral nerve repair 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