{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:57:37Z","timestamp":1777661857482,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,12,30]],"date-time":"2015-12-30T00:00:00Z","timestamp":1451433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Due to their unique properties, adipose derived stem cells (ADSCs) obtain promising potential to enhance nerve regeneration. The aim of this study was to investigate if fibrin\u2010glue embedded ADSCs were a beneficial adjunct to primary coaptation in a rat sciatic nerve model. <jats:italic>Materials and methods<\/jats:italic>: Fifty male Lewis rats underwent sciatic nerve transection and subsequent epineural suture repair. The treatment group received ADSCs re\u2010suspended in fibrin glue, while the control group received fibrin glue only. After 7, 21, 35, and 63 days, analysis involved axon count, myelin sheath thickness as well as N\u2010 and G\u2010ratios. Additionally, muscle weight quotient (operated vs. non\u2010operated site of the same animal) was calculated and compared between treatment and control groups. For co\u2010detection of vital ADSCs, vessel walls, and Schwann cells, immunolabeling was performed with CM\u2010DiI, SMA, and S\u2010100 antibodies, respectively. <jats:italic>Results<\/jats:italic>: ADSCs led to a significant increase of myelinization at day 21 (0.508\u2009\u00b1\u20090.085 \u03bcm vs. 0.381\u2009\u00b1\u20090.044 \u03bcm, <jats:italic>P<\/jats:italic>\u2009=\u20090.025) and day 35 (0.872\u2009\u00b1\u20090.09 \u00b5m vs. 0.495\u2009\u00b1\u20090.078 \u00b5m; <jats:italic>P<\/jats:italic>\u2009=\u20090.01) after surgery. Axon count was significantly increased at day 21 (420\u2009\u00b1\u2009119 vs. 129\u2009\u00b1\u200963; <jats:italic>P<\/jats:italic>\u2009=\u20090.003) and day 63 (284\u2009\u00b1\u2009137 vs. 111\u2009\u00b1\u200926; <jats:italic>P<\/jats:italic>\u2009=\u20090.046) after surgery. N\u2010 and G\u2010ratios were significantly different compared with control indicating enhanced nerve regeneration due to ADSC treatment at each time point (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Muscle weight quotient was significantly higher in the treatment group compared with the control at day 21 (44.01%\u2009\u00b1\u20096.16% vs. 35.03%\u2009\u00b1\u20092.61%; <jats:italic>P<\/jats:italic>\u2009=\u20090.014) and day 63 (65.49%\u2009\u00b1\u20092.81% vs. 58.79%\u2009\u00b1\u20094.06%; <jats:italic>P<\/jats:italic>\u2009=\u20090.009) after surgery. Co\u2010detection of immunolabeled cells showed vital ADSCs at the neuronal repair site and in close proximity to intraneuronal vessels indicating active participation of ADSCs in the process of nerve regeneration and associated angiogenesis. <jats:italic>Conclusion<\/jats:italic>: ADSCs embedded in a fibrin matrix can significantly enhance regeneration of peripheral nerve injuries after primary coaptation. \u00a9 2015 Wiley Periodicals, Inc. Microsurgery 36:491\u2013500, 2016.<\/jats:p><\/jats:sec>","DOI":"10.1002\/micr.30018","type":"journal-article","created":{"date-parts":[[2015,12,30]],"date-time":"2015-12-30T14:11:26Z","timestamp":1451484686000},"page":"491-500","source":"Crossref","is-referenced-by-count":38,"title":["ADSCs in a fibrin matrix enhance nerve regeneration after epineural suturing in a rat model"],"prefix":"10.1002","volume":"36","author":[{"given":"Matthias A.","family":"Reichenberger","sequence":"first","affiliation":[{"name":"ETHIANUM\u2014Clinic for Plastic and Reconstructive Surgery Aesthetic and Preventive Medicine at Heidelberg University Hospital Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolf","family":"Mueller","sequence":"additional","affiliation":[{"name":"University Hospital Leipzig, Department of Neuropathology University of Leipzig Leipzig Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Hartmann","sequence":"additional","affiliation":[{"name":"ETHIANUM\u2014Clinic for Plastic and Reconstructive Surgery Aesthetic and Preventive Medicine at Heidelberg University Hospital Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yannick","family":"Diehm","sequence":"additional","affiliation":[{"name":"BG Trauma Centre Ludwigshafen Clinic for Hand, Plastic and Reconstructive Surgery, Burn Centre, Hand and Plastic Surgery of the University of Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Lass","sequence":"additional","affiliation":[{"name":"Clinical Cooperation Unit Neuropathology German Cancer Research Center Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Koellensperger","sequence":"additional","affiliation":[{"name":"ETHIANUM\u2014Clinic for Plastic and Reconstructive Surgery Aesthetic and Preventive Medicine at Heidelberg University Hospital Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Leimer","sequence":"additional","affiliation":[{"name":"ETHIANUM\u2014Clinic for Plastic and Reconstructive Surgery Aesthetic and Preventive Medicine at Heidelberg University Hospital Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcnter","family":"Germann","sequence":"additional","affiliation":[{"name":"ETHIANUM\u2014Clinic for Plastic and Reconstructive Surgery Aesthetic and Preventive Medicine at Heidelberg University Hospital Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Fischer","sequence":"additional","affiliation":[{"name":"BG Trauma Centre Ludwigshafen Clinic for Hand, Plastic and Reconstructive Surgery, Burn Centre, Hand and Plastic Surgery of the University of Heidelberg Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.30.051606.094337"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-012-0322-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(09)87008-6"},{"key":"e_1_2_6_5_1","first-page":"303","article-title":"Will it be possible to produce peripheral nerves?","volume":"11","author":"Wiberg M","year":"2003","journal-title":"Surg Technol Int"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.main.2014.05.002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2009.09.009"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5356.1528"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00870"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/BA20030173"},{"key":"e_1_2_6_12_1","first-page":"267","article-title":"Fibroblast precursors in normal and irradiated mouse hematopoietic organs","volume":"4","author":"Friedenstein AJ","year":"1976","journal-title":"Exp Hematol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/SAP.0000000000000083"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-6262-6_1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v7.i1.11"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2013.0396"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017899"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/157488810791268564"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-011-9341-8"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-012-9368-5"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2005.14.92"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.1997.tb01313.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1006616"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2752(1996)17:7<348::AID-MICR2>3.0.CO;2-C"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3171\/JNS-07\/12\/1168"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.1920120608"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10006"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-3147.153218"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880141113"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-12700"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2006.12.004"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368909788341289"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20904"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0342"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0308-z"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.5551\/jat.13.77"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2011.24127"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.21099"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surneu.2005.04.022"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368915X688209"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt454"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0000000000000525"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0000000000001321"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240902807026"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2012.02.178"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.22101"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0000000000001822"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000637-198909000-00002"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199808)21:8<1095::AID-MUS20>3.0.CO;2-S"}],"container-title":["Microsurgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmicr.30018","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmicr.30018","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/micr.30018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:59:52Z","timestamp":1695495592000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/micr.30018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,30]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1002\/micr.30018"],"URL":"https:\/\/doi.org\/10.1002\/micr.30018","archive":["Portico"],"relation":{},"ISSN":["0738-1085","1098-2752"],"issn-type":[{"value":"0738-1085","type":"print"},{"value":"1098-2752","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,30]]}}}