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A silicone tube, infused with syngeneic uncultured\u2010SVF was implanted into the facial nerve defect. Groups 1\u20133 received 1 \u00d7 10<jats:sup>3<\/jats:sup>, 1 \u00d7 10<jats:sup>5<\/jats:sup>, and 1 \u00d7 10<jats:sup>7<\/jats:sup> cells, and regenerated nerves were examined at 13 weeks after the surgery. The findings were compared to the autograft and collagen\u2010alone groups with facial palsy score (FPS), the number of myelinated fibers, fiber diameter, axon diameter, myelin thickness, and <jats:italic>g<\/jats:italic> ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was no significant difference in FPS between the autograft and 1 \u00d7 10<jats:sup>5<\/jats:sup>\u2010cell groups at 13 weeks after surgery, and FPS values of these two groups were significantly higher than those of the other three groups (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). Axon diameter significantly increased in the 1 \u00d7 10<jats:sup>5<\/jats:sup>\u2010cell group compared with the 1 \u00d7 10<jats:sup>3<\/jats:sup>\u2010 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and 1 \u00d7 10<jats:sup>7<\/jats:sup>\u2010cell groups (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). Myelin thickness was found to be the highest in the autograft group, followed by the 1 \u00d7 10<jats:sup>5<\/jats:sup>\u2010, 1 \u00d7 10<jats:sup>3<\/jats:sup>\u2010, 1 \u00d7 10<jats:sup>7<\/jats:sup>\u2010cell, and negative control groups, and there were significant differences among all groups (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The infusion of uncultured\u2010SVF into the artificial nerve conduit promoted optimal nerve regeneration that was significantly better than nerve conduit alone. \u00a9 2016 Wiley Periodicals, Inc. Microsurgery 37:808\u2013818, 2017.<\/jats:p><\/jats:sec>","DOI":"10.1002\/micr.30060","type":"journal-article","created":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T13:32:28Z","timestamp":1465219948000},"page":"808-818","source":"Crossref","is-referenced-by-count":24,"title":["Facial\u2010nerve regeneration ability of a hybrid artificial nerve conduit containing uncultured adipose\u2010derived stromal vascular fraction: An experimental study"],"prefix":"10.1002","volume":"37","author":[{"given":"Hajime","family":"Matsumine","sequence":"first","affiliation":[{"name":"Tissue Engineering and Wound Healing Laboratory Division of Plastic Surgery Brigham and Women's Hospital, Harvard Medical School Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Numakura","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital, Harvard Medical School Boston 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