{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:09:06Z","timestamp":1788908946886,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,11,2]],"date-time":"2017-11-02T00:00:00Z","timestamp":1509580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["Kakenhi No.16K10808"],"award-info":[{"award-number":["Kakenhi No.16K10808"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>This study aimed to evaluate the nerve regenerative effect and behavior of Schwann cells (SCs) on local administration of autologous platelet\u2010rich plasma (PRP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010eight Japanese white rabbits were used. A 15\u2010mm sciatic nerve defect was created on the left limb. The resected nerve was used as a reverse autologous nerve. The rabbits were randomly divided into two groups. In group A (<jats:italic>n<\/jats:italic>\u2009=\u200910), only nerve grafting was performed. In group B (<jats:italic>n<\/jats:italic>\u2009=\u200918), nerve grafting was performed with local PRP administration. Right limbs were used as control (group C,<jats:italic>n<\/jats:italic>\u2009=\u200928). The rabbits in each group were equally divided into two subgroups based on the evaluation period of 4 and 12 weeks after grafting. Electrophysiological evaluation, muscle wet\u2010weight, histological evaluation, and multiple immunofluorescence staining were performed to investigate the regenerative effect of PRP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean regenerative axon diameter of the graft portion in group B (2.02\u2009\u00b1\u20090.22\u2010\u03bcm) was significantly larger than that in group A (1.89\u2009\u00b1\u20090.16\u2010\u03bcm) at 4 weeks. The regenerative axon number at the distal portion showed a greater increase in group B (9017\u2009\u00b1\u20092224\/mm<jats:sup>2<\/jats:sup>) than in group A (4955\u2009\u00b1\u20093117\/mm<jats:sup>2<\/jats:sup>) at 12 weeks. Electrophysiological evaluation and muscle wet\u2010weight revealed no significant differences. On immunohistological evaluation, the number of activated SCs increased to a larger extent in group B (188\u2009\u00b1\u200990\/mm<jats:sup>2<\/jats:sup>) than in group A (117\u2009\u00b1\u200951\/mm<jats:sup>2<\/jats:sup>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Local PRP administration increases the regenerative axon diameter and the regenerative axon number at the distal portion. PRP accelerates SC proliferation<jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/micr.30263","type":"journal-article","created":{"date-parts":[[2017,11,2]],"date-time":"2017-11-02T07:57:41Z","timestamp":1509609461000},"page":"300-309","source":"Crossref","is-referenced-by-count":53,"title":["Effect of local administration of platelet\u2010rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6034-1593","authenticated-orcid":false,"given":"Akira","family":"Ikumi","sequence":"first","affiliation":[{"name":"Department of orthopaedic surgery, Faculty of medicine University of Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Hara","sequence":"additional","affiliation":[{"name":"Department of orthopaedic surgery, Faculty of medicine University of Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomokazu","family":"Yoshioka","sequence":"additional","affiliation":[{"name":"Department of orthopaedic surgery, Faculty of medicine University of Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Kanamori","sequence":"additional","affiliation":[{"name":"Department of orthopaedic surgery, Faculty of medicine University of Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"Department of orthopaedic surgery, Faculty of medicine University of Tsukuba Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35240"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2011.0498"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546514525593"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1383(14)70003-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2010.19.5.48045"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.20860"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00330-013-2926-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490200306"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.4.1353"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3171\/FOC.2009.26.2.E5"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2008.37"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2008.11.009"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2008.05.010"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3171\/2010.9.JNS091928"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.PRS.0000138251.07040.51"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1120"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000249726.98801.77"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1085-9489.2003.03029.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290100205"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2010.11.002"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.09.030"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/S0094-1298(20)32661-4","article-title":"Engineering strategies for peripheral nerve repair","volume":"26","author":"Hudson T. 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