{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:45:40Z","timestamp":1792665940342,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T00:00:00Z","timestamp":1537315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005797","name":"Vereniging Trustfonds Erasmus Universiteit Rotterdam","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005797","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008524","name":"University of Montana","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008524","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000871","name":"Mayo Clinic","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A bridging nerve autograft is the gold standard for the repair of segmental nerve injury that cannot be repaired directly. However, limited availability and donor site morbidity remain major disadvantages of autografts. Here, a nerve allograft decellularized with elastase was compared with an autograft regarding functional motor outcome in a rat sciatic segmental nerve defect model. Furthermore, the effect of storage on this allograft was studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty\u2010six Lewis rats (250\u2013300\u2009g) underwent a 10\u2010mm sciatic nerve reconstruction using either a cold\u2010 (<jats:italic>n<\/jats:italic>\u2009=\u200922) or frozen\u2010stored (<jats:italic>n<\/jats:italic>\u2009=\u200922) decellularized nerve allograft or an autograft (<jats:italic>n<\/jats:italic>\u2009=\u200922). Sprague\u2013Dawley rats (300\u2013350\u2009g) served as full major histocompatibility complex\u2010mismatched donors. Functional motor outcome was evaluated after 12 and 16\u2009weeks. Ankle angle, compound muscle action potential (CMAP), isometric tetanic force, wet muscle weight, and histomorphometry were tested bilaterally.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For CMAP and isometric tetanic force, no significant differences were observed between groups. In contrast, for ankle angle, histomorphometry and muscle weight, the cold\u2010stored allograft performed comparable to the autograft, while the frozen\u2010stored allograft performed significantly inferior to the autograft. At week 16, ankle angle was 88.0\u2009\u00b1\u20093.1% in the cold\u2010stored group, 77.4\u2009\u00b1\u20093.6% in the frozen\u2010stored group, and 74.1\u2009\u00b1\u20093.1% in the autograft group (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001); At week 16, the muscle weight showed a recovery up to 71.1\u2009\u00b1\u20094.8% in the autograft group, 67.0\u2009\u00b1\u20096.6% in the cold\u2010stored group, and 64.7\u2009\u00b1\u20093.7% in the frozen\u2010stored group (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A nerve allograft decellularized with elastase, if stored under the right conditions, results in comparable functional motor outcomes as the gold standard, the autograft.<\/jats:p><\/jats:sec>","DOI":"10.1002\/micr.30371","type":"journal-article","created":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T09:35:54Z","timestamp":1537349754000},"page":"772-779","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Comparable functional motor outcomes after repair of peripheral nerve injury with an elastase\u2010processed allograft in a rat sciatic nerve model"],"prefix":"10.1002","volume":"38","author":[{"given":"Caroline A.","family":"Hundepool","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic  Rochester Minnesota"},{"name":"Department of Plastic Reconstructive and Hand Surgery, Erasmus MC, University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liselotte F.","family":"Bulstra","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic  Rochester Minnesota"},{"name":"Department of Plastic Reconstructive and Hand Surgery, Erasmus MC, University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitra","family":"Kotsougiani","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia F.","family":"Friedrich","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven E.R.","family":"Hovius","sequence":"additional","affiliation":[{"name":"Department of Plastic Reconstructive and Hand Surgery, Erasmus MC, University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen T.","family":"Bishop","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander Y.","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Microvascular Research Laboratory, Mayo Clinic  Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,19]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"49","article-title":"Nerve grafting","volume":"133","author":"Berger A.","year":"1978","journal-title":"Clinical Orthopaedics and Related Research"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20975"},{"issue":"7","key":"e_1_2_6_4_1","first-page":"389","article-title":"Epidemiology of trauma at a level 1 trauma center","volume":"73","author":"Campbell B. 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