{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T17:06:55Z","timestamp":1765040815549,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,10,31]],"date-time":"2018-10-31T00:00:00Z","timestamp":1540944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>A basic fibroblast growth factor (bFGF) slow\u2010release system was combined to a biodegradable nerve conduit with the hypothesis this slow\u2010release system would increase the capacity to promote nerve vascularization and Schwann cell proliferation in a rat model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Slow\u2010release of bFGF was determined using Enzyme\u2010Linked ImmunoSorbent Assay (ELISA). A total of 60 rats were used to create a 10 mm gap in the sciatic nerve. A polyglycolic acid\u2010based nerve conduit was used to bridge the gap, either without or with a bFGF slow\u2010release incorporated around the conduit (<jats:italic>n<\/jats:italic>\u2009=\u200930 in each group). At 2 (<jats:italic>n<\/jats:italic>\u2009=\u20096), 4 (<jats:italic>n<\/jats:italic>\u2009=\u20096), 8 (<jats:italic>n<\/jats:italic>\u2009=\u20096), and 20 (<jats:italic>n<\/jats:italic>\u2009=\u200912) weeks after surgery, samples were resected and subjected to histological, immunohistochemical, and transmission electron microscopic evaluation for nerve regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Continuous release of bFGF was found during the observation period of 2 weeks. After in vivo implantation of the nerve conduit, greater endothelial cell migration and vascularization resulted at 2 weeks (proximal: 20.0\u2009\u00b1\u20092.0 vs. 12.7\u2009\u00b1\u20092.1, <jats:italic>P<\/jats:italic>\u2009=\u2009.01, middle: 17.3\u2009\u00b1\u20093.5 vs. 8.7\u2009\u00b1\u20093.2, <jats:italic>P<\/jats:italic>\u2009=\u2009.03). Schwann cells showed a trend toward greater proliferation and axonal growth had significant elongation (4.9\u2009\u00b1\u20091.1 mm vs. 2.8\u2009\u00b1\u20091.5 mm, <jats:italic>P<\/jats:italic>\u2009=\u2009.04) at 4 weeks after implantation. The number of myelinated nerve fibers, indicating nerve maturation, were increased 20\u2009weeks after implantation (proximal: 83.3\u2009\u00b1\u20097.5 vs. 53.3\u2009\u00b1\u20095.5, <jats:italic>P<\/jats:italic>\u2009=\u2009.06, distal: 71.0\u2009\u00b1\u200912.5 vs. 44.0\u2009\u00b1\u200911.1, <jats:italic>P<\/jats:italic>\u2009=\u2009.04).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings suggest that the bFGF slow\u2010release system improves nerve vascularization and Schwann cell proliferation through the biodegradable nerve conduit.<\/jats:p><\/jats:sec>","DOI":"10.1002\/micr.30387","type":"journal-article","created":{"date-parts":[[2018,10,31]],"date-time":"2018-10-31T18:07:23Z","timestamp":1541009243000},"page":"899-906","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A basic fibroblast growth factor slow\u2010release system combined to a biodegradable nerve conduit improves endothelial cell and Schwann cell proliferation: A preliminary study in a rat model"],"prefix":"10.1002","volume":"38","author":[{"given":"Tomokazu","family":"Fukuda","sequence":"first","affiliation":[{"name":"Department of Plastic Reconstructive Surgery Kindai University Faculty of Medicine  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohisa","family":"Kusuhara","sequence":"additional","affiliation":[{"name":"Department of Plastic Reconstructive Surgery Kindai University Faculty of Medicine  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuya","family":"Nakagoshi","sequence":"additional","affiliation":[{"name":"Department of Plastic Reconstructive Surgery Kindai University Faculty of Medicine  Osaka 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