{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:22:00Z","timestamp":1790882520828,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microsurgery"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Traumatic peripheral nerve injury, with an annual incidence reported to be approximately 13\u201323 per 100,000 people, is a serious clinical condition that can often lead to significant functional impairment and permanent disability. Although nerve transfer has become increasingly popular in the treatment of brachial plexus injuries, satisfactory results cannot be obtained even with total nerve root transfer, especially after serious injuries. To overcome this problem, we hypothesize that the application of stem cells in conjunction with nerve transfer procedures may be a viable alternative to more aggressive treatments that do not result in adequate improvement. Similarly, some preliminary studies have shown that adipose stem cells combined with acellular nerve allograft provide promising results in the repair of brachial plexus injury. The purpose of this study was to assess the efficacy of combining adipose\u2010derived stem cells with nerve transfer procedure in a rat brachial plexus injury model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty female Wistar rats weighing 300\u2013350\u2009g and aged 8\u201310\u2009weeks were randomly divided into two groups: a nerve transfer group (NT group) and a nerve transfer combined adipose stem cell group (NT and ASC group). The upper brachial plexus injury model was established by gently avulsing the C5\u2013C6 roots from the spinal cord with microforceps. A nerve transfer from the ulnar nerve to the musculocutaneous nerve (Oberlin procedure) was performed with or without seeded allogeneic adipose tissue\u2010derived stem cells. Adipose tissue\u2010derived stem cells at a rate of 2\u2009\u00d7\u200910<jats:sup>6<\/jats:sup>\u2009cells were injected locally to the surface of the nerve transfer area with a 23\u2010gauge needle. Immunohistochemistry (S100 and PGP 9.5 antibodies) and electrophysiological data were used to evaluate the effect of nerve repair 12\u2009weeks after surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean latency was significantly longer in the NT group (2.0\u2009\u00b1\u20090.0\u2009ms, 95% CI: 1.96\u20132.06) than in the NT and ASC group (1.7\u2009\u00b1\u20090.0\u2009ms, 95% CI: 1.7\u20131.7) (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). The mean peak value was higher in the NT group (1.7\u2009\u00b1\u20090.0\u2009mV, 95% CI: 1.7\u20131.7) than in the NT and ASC group (1.7\u2009\u00b1\u20090.3\u2009mV, 95% CI: 1.6\u20131.9) with no significant difference (<jats:italic>p<\/jats:italic>\u2009=\u2009.61). Although S100 and PGP 9.5 positive areas were observed in higher amounts in the NT and ASC group compared to the NT group, the differences were not statistically significant (<jats:italic>p<\/jats:italic>\u2009=\u2009.26 and .08, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study conducted on rats provides preliminary evidence that adipose\u2010derived stem cells may have a positive effect on nerve transfer for the treatment of brachial plexus injury. Further studies with larger sample sizes and longer follow\u2010up periods are needed to confirm these findings.<\/jats:p><\/jats:sec>","DOI":"10.1002\/micr.31208","type":"journal-article","created":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T13:12:37Z","timestamp":1721135557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Positive effect of ulnar nerve fascicle transfer to musculocutaneous nerve seeded with allogeneic adipose tissue derived stem cells on nerve regeneration for repairing upper brachial plexus injury in a rat model: A preliminary study"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0769-1532","authenticated-orcid":false,"given":"Muhammed Nebil","family":"Selimoglu","sequence":"first","affiliation":[{"name":"Nebil Selimo\u011flu Private Clinic  Tekirda\u011f Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Metin","family":"Kocacan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Histology and Embryology Dumlup\u0131nar University  K\u00fctahya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se\u00e7kin","family":"Tuncer","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Biophysics Osmangazi University  Eski\u015fehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zekeriya","family":"Tosun","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Plastic, Reconstructive and Aesthetic Surgery Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ender","family":"Erdogan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Histology and Embryology Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000197900"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3171\/2013.4.JNS121189"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00939235"},{"key":"e_1_2_12_5_1","volume-title":"The stem cell (K\u00f6k H\u00fccre)","author":"Can A.","year":"2014"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1265023"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-8-110"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/107632700320748"},{"key":"e_1_2_12_9_1","first-page":"1p","volume-title":"Unbiased stereology","author":"Howard C. 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