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Peptide deformylase, a metalloenzyme which catalytically removes N\u2010formyl group from N\u2010terminal methionine of newly synthesized polypeptides, is an important target in antibacterial drug discovery. In this study, we report the structure\u2010based virtual screening of ZINC database in order to discover potential hits as bacterial peptide deformylase enzyme inhibitors with more affinity as compared to GSK1322322, previously known inhibitor. After virtual screening, fifteen compounds of the top hits predicted were purchased and evaluated<jats:italic>in\u2005vitro<\/jats:italic>for their antibacterial activities against one Gram positive (<jats:italic>Staphylococcus aureus<\/jats:italic>) and three Gram negative (<jats:italic>Escherichia coli, Pseudomonas aeruginosa and Klebsiella. pneumoniae<\/jats:italic>) bacteria in different concentrations by disc diffusion method. Out of these, three compounds, ZINC00039650, ZINC03872971 and ZINC00126407, exhibited significant zone of inhibition. The results obtained were confirmed using the dilution method. Thus, these proposed compounds may aid the development of more efficient antibacterial agents.<\/jats:p>","DOI":"10.1002\/minf.201700087","type":"journal-article","created":{"date-parts":[[2017,10,9]],"date-time":"2017-10-09T15:32:40Z","timestamp":1507563160000},"source":"Crossref","is-referenced-by-count":12,"title":["Virtual Screening Approach of Bacterial Peptide Deformylase Inhibitors Results in New Antibiotics"],"prefix":"10.1002","volume":"37","author":[{"given":"Amina","family":"Merzoug","sequence":"first","affiliation":[{"name":"Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences Mentouri Brothers University Constantine 1 \u2013 Algeria"},{"name":"Abdelhafid.Boussouf University Center Mila Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelouahab","family":"Chikhi","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences Mentouri Brothers University Constantine 1 \u2013 Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abderrahmane","family":"Bensegueni","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences Mentouri Brothers University Constantine 1 \u2013 Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanane","family":"Boucherit","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Biochemistry, Department of Biochemistry and Cellular and Molecular Biology, Faculty of Natural and Life Sciences Mentouri Brothers University Constantine 1 \u2013 Algeria"},{"name":"Abdelhafid.Boussouf University Center Mila Algeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sezer","family":"Okay","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences Cankiri Karatekin University Turk"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2006.05.220"},{"key":"e_1_2_7_3_1","first-page":"214","volume":"22","author":"Jaiprakash N. 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