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The present study reports the successful application of ligand\u2010based virtual screening method for the discovery of novel vascular endothelial growth factor receptor\u20102 (VEGFR\u20102) inhibitors. We generated a ligand query model with pharmacophore features from the reported VEGFR\u20102 inhibitors using vROCS tool and performed virtual screening. Among the 2.4 million lead like molecules of ZINC database screened, nineteen prioritized compounds were purchased from Enamine and ChemBridge and tested for VEGFR\u20102 inhibitory activity using Promega's ADP\u2010Glo\u2122 kinase assay. Experimental validation led to the discovery of four compounds <jats:bold>3<\/jats:bold>, <jats:bold>7<\/jats:bold>, <jats:bold>10<\/jats:bold>, and <jats:bold>13<\/jats:bold>. Compound <jats:bold>10<\/jats:bold> exhibited moderate inhibitory activity with the IC<jats:sub>50<\/jats:sub> value of 19.3\u2005\u03bcM. Molecular docking was performed for these compounds and the predicted binding modes reported in this paper may further guide to explore the possible structural changes to get more potent VEGFR\u20102 inhibitors<\/jats:p>","DOI":"10.1002\/minf.201900150","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T03:10:26Z","timestamp":1583723426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Discovery of Vascular Endothelial Growth Factor Receptor\u20102 (VEGFR\u20102) Inhibitors by Ligand\u2010based Virtual High Throughput Screening"],"prefix":"10.1002","volume":"39","author":[{"given":"Chelliah","family":"Selvam","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences Texas Southern University  Houston TX 77004 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotta D.","family":"Mock","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences Texas Southern University  Houston TX 77004 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omana P.","family":"Mathew","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences Texas Southern University  Houston TX 77004 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasturi","family":"Ranganna","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences Texas Southern University  Houston TX 77004 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramasamy","family":"Thilagavathi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Engineering Karpagam Academy of Higher Education  Coimbatore India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,13]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21565"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm301085w"},{"key":"e_1_2_2_4_1","first-page":"123","volume":"2","author":"Fontanella C.","year":"2014","journal-title":"Ann Transl. 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