{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T12:49:49Z","timestamp":1789044589144,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,3,21]],"date-time":"2023-03-21T00:00:00Z","timestamp":1679356800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["mLife"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> is one of the leading nosocomial pathogens that causes both severe acute and chronic infections. The strong capacity of <jats:italic>P. aeruginosa<\/jats:italic> to form biofilms can dramatically increase its antibiotic resistance and lead to treatment failure. The biofilm resident bacterial cells display distinct gene expression profiles and phenotypes compared to their free\u2010living counterparts. Elucidating the genetic determinants of biofilm formation is crucial for the development of antibiofilm drugs. In this study, a high\u2010throughput transposon\u2010insertion site sequencing (Tn\u2010seq) approach was employed to identify novel <jats:italic>P. aeruginosa<\/jats:italic> biofilm genetic determinants. When analyzing the novel biofilm regulatory genes, we found that the cell division factor ZapE (PA4438) controls the <jats:italic>P. aeruginosa pqs<\/jats:italic> quorum sensing system. The \u2206<jats:italic>zapE<\/jats:italic> mutant lost fitness against the wild\u2010type PAO1 strain in biofilms and its production of 2\u2010heptyl\u20103\u2010hydroxy\u20104(1H)\u2010quinolone (PQS) had been reduced. Further biochemical analysis showed that ZapE interacts with PqsH, which encodes the synthase that converts 2\u2010heptyl\u20104\u2010quinolone (HHQ) to PQS. In addition, site\u2010directed mutagenesis of the ATPase active site of ZapE (K72A) abolished the positive regulation of ZapE on PQS signaling. As ZapE is highly conserved among the <jats:italic>Pseudomonas<\/jats:italic> group, our study suggests that it is a potential drug target for the control of <jats:italic>Pseudomonas<\/jats:italic> infections.<\/jats:p>","DOI":"10.1002\/mlf2.12059","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T06:02:53Z","timestamp":1679464973000},"page":"28-42","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cell division factor ZapE regulates <i>Pseudomonas aeruginosa<\/i> biofilm formation by impacting the <i>pqs<\/i> quorum sensing system"],"prefix":"10.1002","volume":"2","author":[{"given":"Xi","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing China"},{"name":"Key University Laboratory of Metabolism and Health of Guangdong, School of Medicine Southern University of Science and Technology Shenzhen China"},{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Center South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minlu","family":"Jia","sequence":"additional","affiliation":[{"name":"Key University Laboratory of Metabolism and Health of Guangdong, School of Medicine Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key University Laboratory of Metabolism and Health of Guangdong, School of Medicine Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Key University Laboratory of Metabolism and Health of Guangdong, School of Medicine Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Cai","sequence":"additional","affiliation":[{"name":"Key University Laboratory of Metabolism and Health of Guangdong, School of Medicine Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoxiao","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Medical Research Center Southern University of Science and Technology Hospital Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3837-6682","authenticated-orcid":false,"given":"Luyan Z.","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianhui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Center South China Agricultural University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7328-154X","authenticated-orcid":false,"given":"Yingdan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key University Laboratory of Metabolism and Health of Guangdong, School of Medicine Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Disease The Second Affiliated Hospital of Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Yang","sequence":"additional","affiliation":[{"name":"Key University Laboratory of Metabolism and Health of Guangdong, School of Medicine Southern University of Science and Technology Shenzhen China"},{"name":"Shenzhen Third People's Hospital, National Clinical Research Center for Infectious Disease The Second Affiliated Hospital of Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10750"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.03317"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux010"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02503.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.23.8066-8073.2004"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5462"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00167"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00043-12"},{"key":"e_1_2_10_10_1","first-page":"27","article-title":"Sociomicrobiology: the connections between quorum sensing and biofilms","volume":"13","author":"Parsek MR","year":"2005","journal-title":"TIM"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-014-0100-x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-020518-120044"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c04731"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07303.x"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009425"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000166"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-7037-8"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-020-0244-x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00930.x"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00022-14"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/004911-0"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504266102"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens7010003"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01010-13"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-00853-2"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.57.030502.090720"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0360072"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.01.044"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2003.10.010"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950170710"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.05008.x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03925"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046718"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058299"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2006.11.014"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06934.x"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3762\/bjoc.14.241"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja3072397"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2009.03160.x"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04402.x"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.4126"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02657"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.11.001"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-018-0076-z"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.115"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0473-0_9"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0473-0_8"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00414-12"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2006\/004911-0"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.167.1.7-11.1986"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9484-5"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-4341-0"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.7b00875"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13114"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.169"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00153"}],"container-title":["mLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.12059","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mlf2.12059","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.12059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T18:06:14Z","timestamp":1692641174000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mlf2.12059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":56,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/mlf2.12059"],"URL":"https:\/\/doi.org\/10.1002\/mlf2.12059","archive":["Portico"],"relation":{},"ISSN":["2770-100X","2770-100X"],"issn-type":[{"value":"2770-100X","type":"print"},{"value":"2770-100X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3]]},"assertion":[{"value":"2022-08-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}