{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T22:50:32Z","timestamp":1773787832217,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T00:00:00Z","timestamp":1727654400000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013262","name":"State Key Laboratory of Respiratory Disease","doi-asserted-by":"publisher","award":["SKLRD\u2010OP\u2010202113"],"award-info":[{"award-number":["SKLRD\u2010OP\u2010202113"]}],"id":[{"id":"10.13039\/100013262","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013262","name":"State Key Laboratory of Respiratory Disease","doi-asserted-by":"publisher","award":["SKLRD\u2010OP\u2010202324"],"award-info":[{"award-number":["SKLRD\u2010OP\u2010202324"]}],"id":[{"id":"10.13039\/100013262","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013262","name":"State Key Laboratory of Respiratory Disease","doi-asserted-by":"publisher","award":["SKLRD\u2010Z\u2010202301"],"award-info":[{"award-number":["SKLRD\u2010Z\u2010202301"]}],"id":[{"id":"10.13039\/100013262","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013262","name":"State Key Laboratory of Respiratory Disease","doi-asserted-by":"publisher","award":["SKLRD\u2010Z\u2010202412"],"award-info":[{"award-number":["SKLRD\u2010Z\u2010202412"]}],"id":[{"id":"10.13039\/100013262","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013262","name":"State Key Laboratory of Respiratory Disease","doi-asserted-by":"publisher","award":["SKLRD\u2010Z\u2010202414"],"award-info":[{"award-number":["SKLRD\u2010Z\u2010202414"]}],"id":[{"id":"10.13039\/100013262","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21920102003"],"award-info":[{"award-number":["21920102003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22037006"],"award-info":[{"award-number":["22037006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82022067"],"award-info":[{"award-number":["82022067"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["mLife"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Treatment of <jats:italic>Mycobacterium abscessus<\/jats:italic> (Mab) infections is very challenging due to its intrinsic resistance to most available drugs. Therefore, it is crucial to discover novel anti\u2010Mab drugs. In this study, we explored an intrinsic resistance mechanism through which Mab resists echinomycin (ECH). ECH showed activity against Mab at a minimum inhibitory concentration (MIC) of 2\u2009\u00b5g\/ml. A \u0394embC strain in which the <jats:italic>embC<\/jats:italic> gene was knocked out showed hypersensitivity to ECH (MIC: 0.0078\u20130.0156\u2009\u00b5g\/ml). The MICs of ECH\u2010resistant strains screened with reference to \u0394embC ranged from 0.25 to 1\u2009\u00b5g\/ml. Mutations in EmbB, including D306A, D306N, R350G, V555I, and G581S, increased the Mab's resistance to ECH when overexpressed in \u0394embC individually (MIC: 0.25\u20130.5\u2009\u00b5g\/ml). These EmbB mutants, edited using the CRISPR\/Cpf1 system, showed heightened resistance to ECH (MIC: 0.25\u20130.5\u2009\u00b5g\/ml). The permeability of these Mab strains with edited genes and overexpression was reduced, as evidenced by an ethidium bromide accumulation assay, but it remained significantly higher than that of the parent Mab. In summary, our study demonstrates that ECH exerts potent anti\u2010Mab activity and confirms that EmbB and EmbC are implicated in Mab's sensitivity to ECH. Mutation in EmbB may partially compensate for a loss of EmbC function.<\/jats:p>","DOI":"10.1002\/mlf2.12139","type":"journal-article","created":{"date-parts":[[2024,10,1]],"date-time":"2024-10-01T03:42:56Z","timestamp":1727754176000},"page":"459-470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["EmbB and EmbC regulate the sensitivity of <i>Mycobacterium abscessus<\/i> to echinomycin"],"prefix":"10.1002","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4769-0473","authenticated-orcid":false,"given":"Jing","family":"He","sequence":"first","affiliation":[{"name":"Institute of Physical Science and Information Technology Anhui University Hefei China"},{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yamin","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyun","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Institute of Marine Drug Guangxi University of Traditional Chinese Medicine Nanning China"},{"name":"CAS Key Laboratory of Tropical Marine Bio\u2010Resources and Ecology RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1993-9547","authenticated-orcid":false,"given":"H. M. Adnan","family":"Hameed","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuiting","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xirong","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingran","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"School of Life Sciences University of Science and Technology of China Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingli","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Ju","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"School of Life Sciences University of Science and Technology of China Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoju","family":"Tan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Chest Hospital Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junying","family":"Ma","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio\u2010Resources and Ecology RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Ju","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Tropical Marine Bio\u2010Resources and Ecology RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxing","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Chest Hospital Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxiong","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Chest Hospital Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-6014","authenticated-orcid":false,"given":"Tianyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Physical Science and Information Technology Anhui University Hefei China"},{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory of Respiratory Infectious Diseases Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"China\u2010New Zealand Joint Laboratory on Biomedicine and Health Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences Guangzhou China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9082541"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr578"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.TNMI7-0027-2016"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0331-1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/2109.141634"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.816-819.2001"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091014-104121"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04183.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03681.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/7.8.1121"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102272200"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2005.10.013"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01825-07"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2022.04.014"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.5578\/mb.20239904"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba9102"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/1061186X.2020.1853733"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.2.326-336.2000"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.02763-21","article-title":"Arabinosyltransferase C mediates multiple drugs intrinsic resistance by altering cell envelope permeability in Mycobacterium abscessus","volume":"10","author":"Wang S","year":"2022","journal-title":"Microbiology Spectrum"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1101\/2023.03.26.534150"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jimb\/kuab079"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01881-19"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1460-0_9"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-35"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00647-09"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02179"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2021.07.035"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450118666170711150034"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.739227"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02809-23"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00947-17"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00298-08"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-11-2649"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9154-9_6"}],"container-title":["mLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.12139","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T03:08:11Z","timestamp":1729739291000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mlf2.12139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/mlf2.12139"],"URL":"https:\/\/doi.org\/10.1002\/mlf2.12139","archive":["Portico"],"relation":{},"ISSN":["2097-1699","2770-100X"],"issn-type":[{"value":"2097-1699","type":"print"},{"value":"2770-100X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"2023-12-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}