{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T21:45:31Z","timestamp":1770155131218,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["mLife"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Among the 14 serovars of\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    (Lm), serovar 4b strains are the most predominant isolates linked to human listeriosis outbreaks\u2010a phenotype associated with their unique wall teichoic acid (WTA) decorated with galactose (Gal) and glucose (Glu). A wealth of knowledge is available for galactosylated\u2010WTA (Gal\u2010WTA) manipulating bacterial homeostasis and virulence, whereas the relationship between glucosylated\u2010WTA (Glu\u2010WTA) and Gal\u2010WTA in listerial physiology and pathogenesis remains unclear. Here, we find that Glu\u2010WTA and Gal\u2010WTA jointly constitute the O\u2010antigen pattern of serovar 4b Lm; however, Glu\u2010WTA specifically serves as the indispensable ligand for listeriophage LP4 adsorption. Moreover, the co\u2010operation between Glu\u2010 and Gal\u2010WTA increases biofilm formation and bacterial resistance to cationic antimicrobial peptide (CRAMP). We further demonstrate that Gal\u2010WTA modulates the anchoring of surface proteins, including IspC, Ami, and InlB. Additionally, dual glycosylated WTA interaction with ActA facilitates bacterial intracellular motility and dissemination. Consistently, Glu\u2010WTA significantly enhances bacterial colonization ability in the mesenteric lymph nodes (MLNs), ileum, liver, and brain of mouse, cooperating with Gal\u2010WTA to facilitate Lm dissemination to distant organs and tissues. In conclusion, we reveal the crucial roles of Glu\u2010WTA in synergizing with Gal\u2010WTA to modulate the integrity of the cell wall structure and exacerbate bacterial infection, providing a global understanding of the hypervirulence and pathogenicity of invasive serovar 4b Lm.\n                  <\/jats:p>","DOI":"10.1002\/mlf2.70041","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T06:40:34Z","timestamp":1765953634000},"page":"638-650","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual glycosylation of wall teichoic acid modulates the O\u2010antigen pattern and virulence in serovar 4b\n                    <i>Listeria monocytogenes<\/i>"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9704-0505","authenticated-orcid":false,"given":"Hao","family":"Yao","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China Yangzhou University Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010product Safety of the Ministry of Education Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuting","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China Yangzhou University Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010product Safety of the Ministry of Education Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruochen","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China Yangzhou University Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010product Safety of the Ministry of Education Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengnan","family":"Dong","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China Yangzhou University Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010product Safety of the Ministry of Education Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhua","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China Yangzhou University Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010product Safety of the Ministry of Education Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luyong","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China Yangzhou University Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010product Safety of the Ministry of Education Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1079-4783","authenticated-orcid":false,"given":"Yuelan","family":"Yin","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China Yangzhou University Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010product Safety of the Ministry of Education Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin'an","family":"Jiao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou University Yangzhou China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China Yangzhou University Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010product Safety of the Ministry of Education Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses Yangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.GPP3-0014-2018"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3501"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.718840"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112371108"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112515"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2022.109572"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-021-00189-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.03.004"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.12.007"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008032"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12072-1"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092412-155620"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01639729"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.813964"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14472"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004919"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13009"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1133-1139.2001"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.2.418-425.1999"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011482"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14455"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14351"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2008.0154"},{"key":"e_1_2_10_25_1","first-page":"179","article-title":"Serovariants of Listeria monocytogenes and other Listeria species","volume":"22","author":"Seeliger HP","year":"1975","journal-title":"Acta Microbiol Acad Sci Hung"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02667-22"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00003-16"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001990"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2311396120"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S445333"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.13.8405"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002891"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9040290"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10071263"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnv229"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01352"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.63.1.174-229.1999"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.582582"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02342-9"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13353"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.12.5309-5319.1997"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.257"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47783-7"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004089"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adj2641"}],"container-title":["mLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.70041","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mlf2.70041","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.70041","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T09:04:49Z","timestamp":1770109489000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mlf2.70041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":44,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/mlf2.70041"],"URL":"https:\/\/doi.org\/10.1002\/mlf2.70041","archive":["Portico"],"relation":{},"ISSN":["2097-1699","2770-100X"],"issn-type":[{"value":"2097-1699","type":"print"},{"value":"2770-100X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"2024-12-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}