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Previous studies showed that\n                    <jats:italic>cis<\/jats:italic>\n                    \u20102\u2010decenoic acid (\n                    <jats:italic>cis<\/jats:italic>\n                    \u2010DA) is involved in the modulation of biofilm dispersion in\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    , but the regulatory mechanism is unclear. Here, we report that\n                    <jats:italic>cis<\/jats:italic>\n                    \u2010DA regulates the physiology and virulence of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    through FadD1, a long\u2010chain fatty acid\u2010CoA ligase.\n                    <jats:italic>cis<\/jats:italic>\n                    \u2010DA specifically binds to FadD1 and enhances the binding ability of FadD1 to the target gene promoter DNA regions. Further analysis showed that FadD1 is a global regulatory factor that controls the transcription of various target genes. Moreover, FadD1 showed catalytic activity on\n                    <jats:italic>cis<\/jats:italic>\n                    \u20102\u2010dodecenoic acid (BDSF) of\n                    <jats:italic>Burkholderia cenocepacia<\/jats:italic>\n                    and enhanced the competitiveness of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    . Together, our work presents a new DSF\u2010type QS signaling system in\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    , which is highlighted by the signal receptor evolved from a canonical enzyme of fatty acid metabolism.\n                  <\/jats:p>","DOI":"10.1002\/mlf2.70044","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T07:11:11Z","timestamp":1766733071000},"page":"623-637","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Cis<\/i>\n                    \u20102\u2010decenoic acid modulates\n                    <i>Pseudomonas aeruginosa<\/i>\n                    virulence through a noncanonical transcriptional regulator"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0258-4065","authenticated-orcid":false,"given":"Shihao","family":"Song","sequence":"first","affiliation":[{"name":"Key Laboratory of 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