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However, clinical translation remains hindered by the lack of rapid and quantitative phage susceptibility testing (PST) platforms capable of evaluating host range, infection potency, and effective multiplicity of infection (MOI). Here, we present a ramanome\u2010based phage susceptibility test (RPST), a phenotypic platform that captures infection\u2010induced remodeling of bacterial macromolecular composition to unify these diagnostic requirements within a single workflow. RPST integrates four Raman biomarkers into a Composite Infection Index (CII), enabling rapid and lysis\u2010independent discrimination between susceptible and resistant bacterial populations within ~1\u2009h, with 96.0% categorical concordance (24\/25) to plaque assays. As a continuous population\u2010level metric, CII quantifies the proportion of infected cells, allowing quantitative ranking of phage potency against shared hosts. By resolving CII trajectories across the MOI and time, RPST further determines the minimal effective MOI, which is the lowest phage\u2010to\u2010bacterium ratio sustaining self\u2010propagating infection, thereby defining the lower boundary for therapeutic feasibility. Together, these capabilities transform PST from static compatibility assays into a dynamic and quantitative framework that bridges\n                    <jats:italic>in vitro<\/jats:italic>\n                    infectivity assessment and infection dynamics relevant to phage therapy.\n                  <\/jats:p>","DOI":"10.1002\/mlf2.70089","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T05:50:36Z","timestamp":1779429036000},"page":"369-382","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid and quantitative phage susceptibility test by ramanome"],"prefix":"10.1002","volume":"5","author":[{"given":"Xiao","family":"Han","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering University of Jinan Jinan China"},{"name":"Single\u2010Cell Center, Shandong Energy Institute, Qingdao New Energy Shandong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofu","family":"Wan","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Diseases, Shenzhen Clinical Medical Research Center for Tuberculosis, Shenzhen Third People's Hospital Southern University of Science and Technology Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Diseases, Shenzhen Clinical Medical Research Center for Tuberculosis, Shenzhen Third People's Hospital Southern University of Science and Technology Shenzhen China"},{"name":"Molecular Biology Research Center &amp; Center for Medical Genetics, School of Life Sciences Central South University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoting","family":"Fu","sequence":"additional","affiliation":[{"name":"Single\u2010Cell Center, Shandong Energy Institute, Qingdao New Energy Shandong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoshan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Single\u2010Cell Center, Shandong Energy Institute, Qingdao New Energy Shandong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Gao","sequence":"additional","affiliation":[{"name":"Single\u2010Cell Center, Shandong Energy Institute, Qingdao New Energy Shandong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao China"},{"name":"Single\u2010Cell Biotechnology Ltd. Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi","family":"Huang","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anle","family":"Ge","sequence":"additional","affiliation":[{"name":"Single\u2010Cell Center, Shandong Energy Institute, Qingdao New Energy Shandong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Qingdao China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiadong","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Jinan Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhou","family":"Lu","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Diseases, 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