{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T22:58:30Z","timestamp":1789081110221,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160036"],"award-info":[{"award-number":["32160036"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360649"],"award-info":[{"award-number":["32360649"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["mLife"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Like many other bacterial organisms,\n                    <jats:italic>Xanthomonas campestris<\/jats:italic>\n                    pv.\n                    <jats:italic>campestris<\/jats:italic>\n                    (\n                    <jats:italic>Xcc<\/jats:italic>\n                    ) employs two motility appendages, a single polar flagellum and type IV pili, for sensing environmental cues and traversing liquids and solid surfaces. However, compared to the flagellum, the molecular mechanisms underlying the function and regulation of the pilus in\n                    <jats:italic>Xcc<\/jats:italic>\n                    and other xanthomonads are not fully understood. In this study, we demonstrated that a special conserved orphan response regulator DrdR, which contains double receiver (REC) domains but lacks an output domain, modulates the activities of motor proteins of pili and regulates bacterial motilities, chemotaxis, and virulence in\n                    <jats:italic>Xcc<\/jats:italic>\n                    . Deletion of\n                    <jats:italic>drdR<\/jats:italic>\n                    in\n                    <jats:italic>Xcc<\/jats:italic>\n                    enhanced the flagellum\u2010dependent swimming motility, inhibited pilus\u2010dependent swarming motility, and altered swimming\u2010chemotaxis. In addition, loss of DrdR led to reduced virulence and hydathode colonization. Protein\u2013protein interaction and enzymatic assays demonstrated that DrdR physically interacts with the pili motor proteins PilB and PilT, enhancing their ATPase activity. Moreover,\n                    <jats:italic>in silico<\/jats:italic>\n                    analysis combined with site\u2010directed mutagenesis revealed that the C\u2010terminal REC domain is a functional receiver, whereas the N\u2010terminal REC domain is a catalytically inactive pseudo\u2010receiver. Taken together, our findings suggest that\n                    <jats:italic>Xcc<\/jats:italic>\n                    DrdR most likely controls pilus\u2010dependent swarming motility through modulating the ATPase activities of two key motor proteins, PilT and PilB, and this motility appears to be vital for the early invasion of\n                    <jats:italic>Xcc<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/mlf2.70092","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:41:06Z","timestamp":1787726466000},"page":"473-485","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tandem receiver domain response regulator DrdR modulates swarming by controlling PilB\/PilT activity in\n                    <i>Xanthomonas campestris<\/i>"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5463-7298","authenticated-orcid":false,"given":"Peidong","family":"Ren","sequence":"first","affiliation":[{"name":"College of Life Science and Technology Guangxi University Nanning China"},{"name":"Plant Protection Research Institute, Guangxi Academy of Agricultural Science Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrou","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijian","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiafeng","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhua","family":"Qi","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiliang","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6974-7266","authenticated-orcid":false,"given":"Rui\u2010Fang","family":"Li","sequence":"additional","affiliation":[{"name":"Plant Protection Research Institute, Guangxi Academy of Agricultural Science Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5082-9755","authenticated-orcid":false,"given":"Guang\u2010Tao","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00626-4"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0195-4"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuab053"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01793-07"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/011320-0"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008448"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009808"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05531.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2008.00495.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2016.01.007"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0361-8"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000935107"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01390-09"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2022112165"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12901"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01152-08"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.1998.11.6.537"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13186"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.70083"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00261-25"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-020518-115931"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2007.04.050"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.060030097"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-1712-3"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-06-14-0184-R"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12920"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.abi9589"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-07-23-0100-R"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-07594-x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00221-23"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14368"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12684"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13117"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.178.12.3462-3469.1996"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13532"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13092013"}],"container-title":["mLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.70092","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mlf2.70092","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.70092","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:27:50Z","timestamp":1788942470000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mlf2.70092"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/mlf2.70092"],"URL":"https:\/\/doi.org\/10.1002\/mlf2.70092","archive":["Portico"],"relation":{},"ISSN":["2097-1699","2770-100X"],"issn-type":[{"value":"2097-1699","type":"print"},{"value":"2770-100X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2025-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}