{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:07:41Z","timestamp":1788944861069,"version":"build-2803163510"},"reference-count":73,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32200141"],"award-info":[{"award-number":["32200141"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["mLife"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    modulates a variety of host cell processes to create a niche permissive for its intracellular replication using effectors injected by its Dot\/Icm type IV secretion system. More than 130 such effectors have been identified, but the function of most of them remains unknown. Using an activity\u2010based screening strategy, we identified the\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    protein CubL1 (Cbu0295) as an E3 ubiquitin ligase. Further analysis reveals that CubL1 catalyzes ubiquitination of eIF3g, a dual\u2010function protein involved in apoptosis and protein translation initiation. CubL1\u2010induced monoubiquitination inhibits the nuclease activity of eIF3g, essential for its role in cell death execution. Host cells infected by a\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    mutant lacking\n                    <jats:italic>cubL1<\/jats:italic>\n                    became more susceptible to cell death induction. Thus,\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    employs multiple distinct mechanisms to maintain host cell viability to ensure successful completion of its intracellular life cycle.\n                  <\/jats:p>","DOI":"10.1002\/mlf2.70093","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T07:32:37Z","timestamp":1787815957000},"page":"432-446","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Coxiella burnetii<\/i>\n                    inhibits host cell death by ubiquitination\u2010induced inactivation of an apoptosis nuclease"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2190-5867","authenticated-orcid":false,"given":"Yong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Jiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuxin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingya","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao\u2010Tao","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbial Pathogenesis and Interventions of Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education College of Life Sciences, Fujian Normal University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songying","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbial Pathogenesis and Interventions of Fujian Province University, the Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of the Ministry of Education College of Life Sciences, Fujian Normal University Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao\u2010Qing","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Center of Infectious Diseases and Pathogen Biology, Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases The First Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00687-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.147"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00804-1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0802.010164"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.4.1251-1258.1993"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.3.5.577"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00226-11"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00406-10"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00188"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mlf2.70058"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02763-14"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1728098"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1914892117"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2110877119"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10205"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004380107"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12066"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010266"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13305"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2308752120"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201902074"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2016.30"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2217602120"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2013.09.027"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060815-014922"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00071-13"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017638"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2016.35"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1514568112"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00370"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1402605111"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160719"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201540620"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2006.08.005"},{"key":"e_1_2_10_38_1","first-page":"612","article-title":"Eukaryotic translation initiation factor 3 subunit G promotes human colorectal cancer","volume":"11","author":"Yang C","year":"2019","journal-title":"Am J Transl Res"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004286"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01301.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(89)90263-0"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(87)90129-X"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002820-199104000-00001"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01204-13"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2016.1139280"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad763"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2011.07.003"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.790442"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010485107"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3049"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2000-1-5-reviews3002"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.91"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.15210"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20167381"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001216"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.67"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-4315-1_11"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00223-2"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-4315-1_13"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00175-11"},{"key":"e_1_2_10_61_1","article-title":"Generation and multi\u2010phenotypic high\u2010content screening of Coxiella burnetii transposon mutants","volume":"99","author":"Martinez E","year":"2015","journal-title":"J Vis Exp"},{"key":"e_1_2_10_62_1","article-title":"Identification of essential genes in Coxiella burnetii","volume":"9","author":"Metters G","year":"2023","journal-title":"Microb Genom"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19970315)13:3<233::AID-YEA88>3.0.CO;2-E"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00824-19"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.1869-1874.2003"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-018-5915-6"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.102032"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.468"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202051163"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000822"},{"key":"e_1_2_10_71_1","doi-asserted-by":"crossref","DOI":"10.17226\/25801","volume-title":"Guide for the care and use of laboratory animals","author":"National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals","year":"2011"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.38.1.41"},{"key":"e_1_2_10_73_1","volume-title":"Using antibodies: a laboratory manual","author":"Harlow E","year":"1999"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-50311-2"}],"container-title":["mLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.70093","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mlf2.70093","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mlf2.70093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:27:46Z","timestamp":1788942466000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mlf2.70093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":73,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/mlf2.70093"],"URL":"https:\/\/doi.org\/10.1002\/mlf2.70093","archive":["Portico"],"relation":{},"ISSN":["2097-1699","2770-100X"],"issn-type":[{"value":"2097-1699","type":"print"},{"value":"2770-100X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}