{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T12:41:47Z","timestamp":1792586507622,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42173079"],"award-info":[{"award-number":["42173079"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["mLife"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Currently, the production of high\u2010value chemicals from natural lignocellulose is of great significance for a circular economy. In this study, a robust platform was developed based on\n                    <jats:italic>Sphingomonas sanxanigenens<\/jats:italic>\n                    NXdP, which co\u2010utilizes glucose and xylose to synthesize polysaccharide sanxan, for efficient bioconversion of lignocellulose\u2010derived sugars. Sanxan is a promising high\u2010value product due to its high viscosity at low concentrations, excellent acid\u2010gelling capabilities, and unique rheological characteristics. Through a cell growth\u2010based screening strategy and gene manipulation, a key transporter (0233) required for the uptake of both glucose and xylose was identified, clarifying the co\u2010utilization mechanism. Additionally, the metabolic pathways of glucose and xylose were individually blocked to assess their contributions and activities. Furthermore, the metabolic network of strain NXdP was reprogrammed via a \u201cflux\u2010redirection\u201d strategy to develop a potential microbial host with improved sanxan production on mixed sugars. The final engineered strain S09 achieved a sanxan titer of 20.25\u2009\u00b1\u20090.78\u2009g\/kg under mixed\u2010sugar fermentation, representing a 13.51%\u2009\u00b1\u20094.38% increase compared with the original strain NXdP. The mechanism underlying this enhanced production was revealed by central carbon metabolism (CCM) analysis. Overall, this study provides insights into the efficient conversion of lignocellulose\u2010derived sugars and offers genetic components for the green production of other high\u2010value\u2010added products.\n                  <\/jats:p>","DOI":"10.1002\/mlf2.70110","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T11:56:43Z","timestamp":1792583803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clarification and reprogramming of glucose and xylose metabolism in\n                    <i>Sphingomonas sanxanigenens<\/i>\n                    for efficient sanxan biosynthesis"],"prefix":"10.1002","author":[{"given":"Yue","family":"Ming","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuangzhuang","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiting","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jue","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weilong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Sha","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Food Science Tianjin University of Commerce Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqiang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengmeng","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Food Science Tianjin University of Commerce Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5367-1914","authenticated-orcid":false,"given":"Ting","family":"Ma","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ymben.2022.01.006","article-title":"Systems metabolic engineering of Corynebacterium glutamicum for high\u2010level production of 1,3\u2010propanediol from glucose and xylose","volume":"70","author":"Li Z","year":"2022","journal-title":"Metab Eng"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1039\/D1SE00927C","article-title":"Valorisation of xylose to renewable fuels and chemicals, an 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