{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:44:48Z","timestamp":1791614688008,"version":"4.3.4"},"reference-count":71,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,11,11]],"date-time":"2004-11-11T00:00:00Z","timestamp":1100131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the virulence properties and clonal relationship of 21<jats:italic> Escherichia coli<\/jats:italic> strains of serogroup O78 isolated from diarrhoeic cattle and calves. Isolates were screened for 18 genes representing virulence features of different<jats:italic> Escherichia coli <\/jats:italic>pathotypes. None of the strains harboured enterotoxin\u2010genes<jats:italic> estIa\/Ib<\/jats:italic>,<jats:italic> eltIa<\/jats:italic>\/<jats:italic>Ib,<\/jats:italic> or Shiga toxin (<jats:italic>stx<\/jats:italic>) genes, genes involved in adhesion (<jats:italic>eae<\/jats:italic>,<jats:italic> f5<\/jats:italic>,<jats:italic> f41<\/jats:italic>) hemolysin gene<jats:italic> hlyA<\/jats:italic> or invasion gene<jats:italic> ipaC<\/jats:italic>. With a high prevalence we detected enterotoxin <jats:italic>astA<\/jats:italic> (61.9%), genes involved in iron acquisition, like<jats:italic> fyuA<\/jats:italic>,<jats:italic> irp<\/jats:italic> (each 57.1%) and<jats:italic> iucD <\/jats:italic>(81.0%), and the operon sequence of Colicin V plasmids (38.1%). Some strains possessed toxin genes<jats:italic> cdt\u2010IIIB<\/jats:italic> and <jats:italic>cnf1\/2<\/jats:italic> (both 14.3%), the invasion gene <jats:italic>tia<\/jats:italic> (23.8%), and the serine protease encoding gene <jats:italic>espP<\/jats:italic> (23.8%). Moreover, we could show that <jats:italic>E. coli<\/jats:italic> O78 strains under investigation were able to adhere to and invade MDBK\u2010cells with varying efficiencies. The results indicate that the closely related O78 strains, constituting two major PFGE\u2010clusters, harbor various virulence features for bovine intestinal disease but cannot be grouped into one of the common<jats:italic> E. coli<\/jats:italic> intestinal pathogenic or other pathotypes according to their virulence gene pattern. Nevertheless, the ability to adhere, invade or harbor toxin genes lets us suggest that O78 strains isolated from diarrheal cases in bovines urges further investigations on the zoonotic potential of these strains.<\/jats:p>","DOI":"10.1002\/mnfr.200400063","type":"journal-article","created":{"date-parts":[[2004,11,11]],"date-time":"2004-11-11T09:18:50Z","timestamp":1100164730000},"page":"504-514","source":"Crossref","is-referenced-by-count":16,"title":["Molecular characteristics of <i>Escherichia coli<\/i> serogroup O78 strains isolated from diarrheal cases in bovines urge further investigations on their zoonotic potential"],"prefix":"10.1002","volume":"48","author":[{"given":"Christa","family":"Ewers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Sch\u00fcffner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Wei\u00df","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Baljer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lothar H.","family":"Wieler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,11,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(85)80814-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.3.4.345"},{"key":"e_1_2_1_4_2","unstructured":"Sobjinski G. 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