{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:38:23Z","timestamp":1788176303336,"version":"build-2803163510"},"reference-count":12,"publisher":"Wiley","issue":"4-5","license":[{"start":{"date-parts":[[2006,3,20]],"date-time":"2006-03-20T00:00:00Z","timestamp":1142812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Numerous studies have demonstrated the potential of isoflavones occurring in soy (<jats:italic>Glycine max <\/jats:italic>L.) and red clover (<jats:italic>Trifolium pratense <\/jats:italic>L.) to alleviate climacteric complaints. They have also shown beneficial effects on the cardiovascular system and in the prevention of osteoporosis. As a result, several companies offer nutraceuticals based on soy or red clover extracts. The aim of the present study was the isolation of pure isoflavones on a preparative scale, in order to obtain standards for biological tests and for the quantification of isoflavones in nutraceuticals and foods. High\u2010speed countercurrent chromatography, a special type of liquid\u2013liquid partition chromatography, was applied to the preparative isolation of isoflavones. By using this technique the major monoglucosylated and acetylated isoflavones from soy extracts were obtained after a cleaning\u2010up step on Amberlite XAD\u20107 material. Furthermore, it was possible to isolate isoflavone aglycones as well as glycosides from a red clover extract. Purity and identity of the isolated isoflavones were confirmed by HPLC with DAD, HPLC\u2010ESI multiple\u2010MS, and NMR spectroscopy.<\/jats:p>","DOI":"10.1002\/mnfr.200500206","type":"journal-article","created":{"date-parts":[[2006,3,20]],"date-time":"2006-03-20T10:47:43Z","timestamp":1142851663000},"page":"356-361","source":"Crossref","is-referenced-by-count":19,"title":["Preparative isolation of isoflavones from soy and red clover"],"prefix":"10.1002","volume":"50","author":[{"given":"Melanie","family":"St\u00fcrtz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Lander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Winterhalter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,6]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf00044a016"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf00035a027"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.08.001"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<1044::AID-JSFA586>3.0.CO;2-N"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/68.6.1333S"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.55.031903.141729"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/129.3.758S"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(01)00612-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00215-5"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","unstructured":"Degenhardt A. Habben S. Winterhalter P. in: Shahidi F. Weerasinghe D. K. (Eds.) Nutraceutical Beverages. Chemistry Nutrition and Health Effects ACS Symposium Series 871 American Chemical Society Washington DC 2004 pp. 443\u2013456.","DOI":"10.1021\/bk-2004-0871.ch031"},{"key":"e_1_2_1_12_2","unstructured":"Ito Y. Conway W. D.(Eds.) High\u2010Speed Countercurrent Chromatography Wiley\u2010Interscience New York 1996."},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002170100386"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.200500206","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.200500206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T04:22:17Z","timestamp":1693369337000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.200500206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4]]},"references-count":12,"journal-issue":{"issue":"4-5","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["10.1002\/mnfr.200500206"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.200500206","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4]]}}}