{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:43:07Z","timestamp":1792802587824,"version":"4.5.0"},"reference-count":15,"publisher":"Wiley","issue":"4-5","license":[{"start":{"date-parts":[[2006,3,13]],"date-time":"2006-03-13T00:00:00Z","timestamp":1142208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The glycoprotein Wnt\u20107a participates in a signaling pathway that transmits information among uterine cell types. Disruption of this pathway by the transplacentally acting carcinogen diethylstilbestrol (DES) is associated with morphological abnormalities of the female reproductive tract (FRT). This raises the question whether estrogens in the diet might also interfere with this pathway. Therefore, this study investigated the influence of the steroid hormone 17\u03b2\u2010estradiol (E2), the mycotoxin zearalenone (ZEN), the soy phytoestrogen genistein (GEN), and DES on the expression of Wnt\u20107a in an endometrial adenocarcinoma cell line (Ishikawa cells) by reverse transcription\/competitive PCR. In addition, the enzymatic activity of alkaline phosphatase (ALP) was determined, which is estrogen receptor (ER)\u2010dependently regulated in Ishikawa cells. After treatment of Ishikawa cells with E2, ZEN, GEN, and DES, a decrease in the gene expression of Wnt\u20107a was observed. Maximum effect (50% reduction) was observed after treatment with concentrations that induced maximum expression of the ALP. Experiments in the presence of the ER antagonist (ICI 182,780) suggested that the ER is involved in the regulation of Wnt\u20107a in Ishikawa cells. In conclusion, interference with the expression of Wnt genes in the FRT might be a novel mechanism by which estrogens disrupt the function of the FRT.<\/jats:p>","DOI":"10.1002\/mnfr.200500215","type":"journal-article","created":{"date-parts":[[2006,3,13]],"date-time":"2006-03-13T14:46:44Z","timestamp":1142261204000},"page":"368-372","source":"Crossref","is-referenced-by-count":35,"title":["Estrogens modulate the gene expression of Wnt\u20107a in cultured endometrial adenocarcinoma cells"],"prefix":"10.1002","volume":"50","author":[{"given":"J\u00f6rg","family":"Wagner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leane","family":"Lehmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,6]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.20.010403.113126"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"3201","DOI":"10.1242\/dev.125.16.3201","volume":"125","author":"Miller C.","year":"1998","journal-title":"Development"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20121"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0413"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-127-6-2757"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-0774.2002.tb00105.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2005.02.011"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1056-8719(97)00078-6"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/3027"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-9007(00)00333-6"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.3.552S"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)09480-9"},{"key":"e_1_2_1_14_2","first-page":"4325","volume":"61","author":"Newbold R. R.","year":"2001","journal-title":"Cancer Res."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-6238(01)00108-3"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1080\/0265203031000094645"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.200500215","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.200500215","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T04:22:50Z","timestamp":1693369370000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.200500215"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4]]},"references-count":15,"journal-issue":{"issue":"4-5","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["10.1002\/mnfr.200500215"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.200500215","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4]]}}}