{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T23:56:07Z","timestamp":1789602967789,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,9,14]],"date-time":"2009-09-14T00:00:00Z","timestamp":1252886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Opisthorchis viverrini<\/jats:italic> (OV) infection is endemic in northeastern Thailand. We have previously reported that OV infection induces oxidative and nitrative DNA damage<jats:italic> via <\/jats:italic>chronic inflammation, which contributes to the disease and cholangiocarcinogenesis. Here, we examined the effect of curcumin, an antioxidant, on pathogenesis in OV\u2010infected hamsters. DNA lesions were detected by double immunofluorescence and the hepatic expression of oxidant\u2010generating and antioxidant genes was assessed by quantitative RT\u2010PCR analysis. Dietary 1.0% curcumin significantly decreased OV\u2010induced accumulation of 8\u2010oxo\u20107,8\u2010dihydro\u20102\u2032\u2010deoxyguanosine (8\u2010oxodG), an oxidative DNA lesion, and 8\u2010nitroguanine, a nitrative DNA lesion, in the nucleus of bile duct epithelial and inflammatory cells. Expression of oxidant\u2010generating genes (inducible nitric oxide synthase; iNOS, its nuclear transcriptional factor, NF\u2010\u03baB, and cyclooxygenase\u20102), and plasma levels of nitrate, malondialdehyde, and alanine aminotransferase, were also decreased in curcumin\u2010treated group. In contrast, curcumin increased the mRNA expression of antioxidant enzymes (Mn\u2010superoxide dismutase and catalase), and ferric\u2010reducing anti\u2010oxidant power in the plasma. In conclusion, curcumin reduced oxidative and nitrative DNA damage by suppression of oxidant\u2010generating genes and enhancement of antioxidant genes, leading to inhibition of oxidative and nitrative stress. Therefore, curcumin may be used as a chemopreventive agent to reduce the severity of OV\u2013associated diseases and the risk of cholangiocarcinoma (CCA).<\/jats:p>","DOI":"10.1002\/mnfr.200800567","type":"journal-article","created":{"date-parts":[[2009,9,14]],"date-time":"2009-09-14T18:15:29Z","timestamp":1252952129000},"page":"1316-1328","source":"Crossref","is-referenced-by-count":64,"title":["Curcumin reduces oxidative and nitrative DNA damage through balancing of oxidant\u2013antioxidant status in hamsters infected with <i>Opisthorchis viverrini<\/i>"],"prefix":"10.1002","volume":"53","author":[{"given":"Somchai","family":"Pinlaor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puangrat","family":"Yongvanit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suksanti","family":"Prakobwong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Butsara","family":"Kaewsamut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarinya","family":"Khoontawad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Porntip","family":"Pinlaor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Hiraku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,10,2]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"IARC Infection with liver flukes (Opisthorchis viverrini Opisthorchis felineus Clornorchis sinensis). IARC monographs on the Evaluation of Carcinogenic Risks to Human.International Agency for Research on Cancer Lyon.1994 61 121\u2013175."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2003.01.002"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040201"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(94)90245-3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2006.049"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01322"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1046"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(02)00506-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2004.08.004"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgh157"},{"key":"e_1_2_1_12_2","first-page":"118","article-title":"The role of oxygen radicals in human disease, with particular reference to the vascular system","author":"Halliwell B.","year":"1993","journal-title":"Haemostasis"},{"key":"e_1_2_1_13_2","first-page":"H1115","article-title":"Role of intracellular SOD in oxidant\u2010induced injury to normal and copper\u2010deficient cardiac myocytes","volume":"268","author":"Sarvazyan N.","year":"1995","journal-title":"Am. 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