{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T20:52:57Z","timestamp":1696798377498},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,9,14]],"date-time":"2009-09-14T00:00:00Z","timestamp":1252886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The emergence of prion diseases in cattle during the bovine spongiform encephalopathy (BSE) epidemic and the transmission to humans causing variant Creutzfeldt\u2013Jakob disease by consume of BSE\u2010contaminated meat has focused attention on the use of tissues from the central nervous system (CNS) in food. To avoid food contamination, it is regulated by law that specified risk material has to be removed from food chains. Detection of well\u2010expressed CNS indicator proteins such as the glial fibrillary acidic protein (GFAP) assumes an important role in detection of food contamination; however, the sensitivity of detection performed on basis of ELISAs is limited. Consequently, there is an urgent need for high\u2010sensitivity detection methods. We describe the development of an immuno\u2010PCR assay with enhancement of the immunoreaction by amplification of a DNA fragment linked to the antigen\u2013antibody complex. This immuno\u2010PCR assay shows enhanced sensitivity in GFAP detection of at least two orders of magnitude compared to the ELISA technique carried out under identical conditions. The immuno\u2010PCR proves to be a suitable tool for the high\u2010sensitivity detection of marker proteins in food imported by food chain processes. It is applicable for use by food and veterinary facilities, and institutes involved in food monitoring.<\/jats:p>","DOI":"10.1002\/mnfr.200800587","type":"journal-article","created":{"date-parts":[[2009,9,14]],"date-time":"2009-09-14T18:15:47Z","timestamp":1252952147000},"page":"1329-1335","source":"Crossref","is-referenced-by-count":7,"title":["High sensitivity detection of the glial fibrillary acidic protein as indicator for TSE risk material in meat products using an immuno\u2010PCR"],"prefix":"10.1002","volume":"53","author":[{"given":"Thorsten","family":"Kuczius","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karsten","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helge","family":"Karch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenlan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,10,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/39057"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/38925"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13363"},{"key":"e_1_2_1_5_2","first-page":"461","article-title":"Experimental scrapie in the mouse: a review of an important model disease.","volume":"63","author":"Kimberlin R.\u2010H.","year":"1976","journal-title":"Sci Prog."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.40.3_Part_1.513"},{"key":"e_1_2_1_7_2","first-page":"537","article-title":"Immunodetection of a disease specific PrP fraction in scrapie\u2010affected sheep and BSE\u2010affected cattle.","volume":"131","author":"Mohri S.","year":"1992","journal-title":"Vet. 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