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This study investigated the molecular mechanism of these effects of vitamin B6.<\/jats:p><jats:p><jats:bold>Methods and results:<\/jats:bold> DNA microarray analysis was used to obtain information on changes in colon gene expression from vitamin B6 (pyridoxine) repletion in vitamin B6\u2010deficient rats. Pyridoxine supplementation down\u2010regulated the inflammatory molecule, serine protease inhibitor clade A member 3 (SPI\u20103) mRNA expression in the colon. This study also showed that tumor necrosis factor \u03b1 (TNF\u2010\u03b1) induced SPI\u20103 mRNA expression in HT\u201029 human colon cancer cells, and vitamin B6 (pyridoxal hydrochloride) pretreatment of HT\u201029 cells inhibited TNF \u2010induced mRNA expression of SPI\u20103. Vitamin B6 inhibited TNF\u2010\u03b1\u2010induced NF\u2010\u03baB activation via suppression of I\u03baB\u03b1 degradation in HT\u201029 cells. HT\u201029 cells stably expressing epitope\u2010tagged ubiquitin were generated and vitamin B6 pretreatment was shown to inhibit ubiquitination of the IkB protein in response to TNF\u2010\u03b1\u2010i.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Vitamin B6 suppressed SPI\u20103 expression in the colon of rats and in TNF\u2010\u03b1\u2010stimulated HT\u201029 cells. Further, this study showed a possible role of vitamin B6 in the regulation of protein ubiquitination.<\/jats:p>","DOI":"10.1002\/mnfr.201000282","type":"journal-article","created":{"date-parts":[[2011,1,5]],"date-time":"2011-01-05T10:11:18Z","timestamp":1294222278000},"page":"635-643","source":"Crossref","is-referenced-by-count":14,"title":["Vitamin B6 suppresses serine protease inhibitor 3 expression in the colon of rats and in TNF\u2010\u03b1\u2010stimulated HT\u201029 cells"],"prefix":"10.1002","volume":"55","author":[{"given":"Noriyuki","family":"Yanaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomomi","family":"Ohata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keigo","family":"Toya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Kanda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsuko","family":"Hirata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norihisa","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,1,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0950344"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/103.1.123"},{"key":"e_1_2_6_4_2","volume-title":"What We Eat in America, NHANES 2001\u20132002: Usual Nutrient Intakes from Food Compared to Dietary Reference Intakes","author":"Moshfegh A. 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