{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:45:51Z","timestamp":1791265551952,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,3,24]],"date-time":"2011-03-24T00:00:00Z","timestamp":1300924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Scope<\/jats:bold>: DNA methylation patterns are tissue specific and may influence tissue\u2010specific gene regulation. Human studies investigating DNA methylation in relation to environmental factors primarily use blood\u2010derived DNA as a surrogate for DNA from target tissues. It is therefore important to know if DNA methylation changes in blood in response to environmental changes reflect those in target tissues. Folate intake can influence DNA methylation, via altered methyl donor supply. Previously, manipulations of maternal folate intake during pregnancy altered the patterns of DNA methylation in offspring but, to our knowledge, the consequences for maternal DNA methylation are unknown. Given the increased requirement for folate during pregnancy, mothers may be susceptible to aberrant DNA methylation due to folate depletion. <jats:bold>Methods and results<\/jats:bold>: Female mice were fed folate\u2010adequate (2\u2009mg folic acid\/kg diet) or folate\u2010deplete (0.4\u2009mg folic acid\/kg diet) diets prior to mating and during pregnancy and lactation. Following weaning, dams were killed and DNA methylation was assessed by pyrosequencing<jats:sup>\u00ae<\/jats:sup> in blood, liver, and kidney at the <jats:italic>Esr1<\/jats:italic>, <jats:italic>Igf2<\/jats:italic> differentially methylated region (DMR)1, <jats:italic>Igf2<\/jats:italic> DMR2, <jats:italic>Slc39a4<\/jats:italic>CGI1, and <jats:italic>Slc39a4<\/jats:italic>CGI2 loci. We observed tissue\u2010specific differences in methylation at all loci. Folate depletion reduced <jats:italic>Igf2<\/jats:italic> DMR1 and <jats:italic>Slc39a4<\/jats:italic>CGI1 methylation across all tissues and altered <jats:italic>Igf2<\/jats:italic> DMR2 methylation in a tissue\u2010specific manner (<jats:italic>p<\/jats:italic>&lt;0.05). <jats:bold>Conclusion<\/jats:bold>: Blood\u2010derived DNA methylation measurements may not always reflect methylation within other tissues. Further measurements of blood\u2010derived and tissue\u2010specific methylation patterns are warranted to understand the complexity of tissue\u2010specific responses to altered nutritional exposure.<\/jats:p>","DOI":"10.1002\/mnfr.201100008","type":"journal-article","created":{"date-parts":[[2011,3,24]],"date-time":"2011-03-24T15:20:16Z","timestamp":1300980016000},"page":"1026-1035","source":"Crossref","is-referenced-by-count":56,"title":["Blood as a surrogate marker for tissue\u2010specific DNA methylation and changes due to folate depletion in post\u2010partum female mice"],"prefix":"10.1002","volume":"55","author":[{"given":"Jill A.","family":"McKay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi K.","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianne","family":"Ford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Mathers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1655"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.947102"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000602"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060022"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.096826.109"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2010.00577.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/153537020422901002"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/bdrc.20163"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.299.24.2877"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddm010"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-9173-5_13"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1201\/9781420063561.ch8"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/128.7.1204"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/72.4.998"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.062066299"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602439"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.07.077"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.6.1382"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114507894438"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20230"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.15.5293-5300.2003"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.11.949"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007845"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi484"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0330709"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00084.2006"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1586\/erm.09.53"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-009-9216-2"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1201\/9781420004847.ch2"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2009.080072"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-08-0703"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq126"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.4161\/epi.6.1.13391"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/65.1.46"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/24.1.81"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2008.01.010"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.931569"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.10.2653"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1017\/S002966511000008X"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/em.20366"}],"container-title":["Molecular Nutrition &amp; 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