{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T04:15:42Z","timestamp":1780719342871,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,7,19]],"date-time":"2011-07-19T00:00:00Z","timestamp":1311033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"\u2018The European Nutrigenomics Organisation; linking genomics, nutrition and health research\u2019, NuGO","award":["CT-2004-505944"],"award-info":[{"award-number":["CT-2004-505944"]}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"crossref","award":["BB\/G007993\/1"],"award-info":[{"award-number":["BB\/G007993\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Scope<\/jats:bold>: Epidemiological evidence supports the developmental origins of health and disease hypothesis that developmental under\/over\u2010nutrition increases adulthood disease risk. Epigenetic markings are one potential mechanism mediating these effects. Altered folate supply may influence methyl group availability for DNA methylation. We reported low folate supply in utero was associated with reduced global DNA methylation in the murine small intestine of adult offspring. We hypothesised that aberrant methylation would be observed during early development.<\/jats:p><jats:p><jats:bold>Methods and results<\/jats:bold>: Female C57BL\/6J mice were fed diets containing 2\u2009mg folic acid\/kg or 0.4\u2009mg folic acid\/kg 4\u2009wk before mating and during pregnancy. At 17.5 day gestation, gene methylation in fetal gut was analysed by Pyrosequencing<jats:sup>\u00ae<\/jats:sup>. Low folate reduced overall methylation of <jats:italic>Slc394a<\/jats:italic> by 3.4% (<jats:italic>p<\/jats:italic>=0.038) but did not affect <jats:italic>Esr1<\/jats:italic> or <jats:italic>Igf2<\/jats:italic> differentially methylated region (DMR) 1. There were sex\u2010specific differences in <jats:italic>Slc394a<\/jats:italic> and <jats:italic>Esr1<\/jats:italic> methylation (2.4% higher in females (<jats:italic>p<\/jats:italic>=0.002); 4% higher in males (<jats:italic>p<\/jats:italic>=0.0014), respectively).<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold>: This is the first study reporting causal effects of maternal folate depletion on gene\u2010specific methylation in fetal gut. These observations support reports that altered methyl donor intake during development affects DNA methylation in the offspring. The consequences of epigenetic changes for health throughout the life course remain to be investigated.<\/jats:p>","DOI":"10.1002\/mnfr.201100150","type":"journal-article","created":{"date-parts":[[2011,7,19]],"date-time":"2011-07-19T10:43:43Z","timestamp":1311072223000},"page":"1717-1723","source":"Crossref","is-referenced-by-count":41,"title":["Maternal folate supply and sex influence gene\u2010specific DNA methylation in the fetal gut"],"prefix":"10.1002","volume":"55","author":[{"given":"Jill A.","family":"McKay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi K.","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline L.","family":"Relton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianne","family":"Ford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Mathers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,7,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2004.1518"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02725"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000082311"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-380864-6.00001-8"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748\u20101716.2011.02278.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.947102"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.8700"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/135.6.1382"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.15.5293-5300.2003"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20230"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2008.01.010"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007845"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12263-010-0199-1"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1748"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007951"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0330709"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201100008"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-08-0703"},{"key":"e_1_2_6_20_2","first-page":"6442","article-title":"Loss of imprinting in colorectal cancer linked to hypomethylation of H19 and IGF2","volume":"62","author":"Cui H.","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006555"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.187682"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.07.126"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/123.11.1939"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2006.085"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1081"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90186-I"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1136\/mp.54.4.206"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707258104"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-0557"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709307104"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013158"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-2405"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-005-1317-9"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-007-0430-3"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006767"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2009.11.002"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665108008744"}],"container-title":["Molecular Nutrition &amp; 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