{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:57:27Z","timestamp":1792198647299,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T00:00:00Z","timestamp":1323388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education and Science, Republic of Serbia","award":["172049"],"award-info":[{"award-number":["172049"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Scope<\/jats:bold>\n                    Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of \u03b2\u20101,3\u2010glucanase from banana (\n                    <jats:italic>Musa acuminata<\/jats:italic>\n                    ), denoted as Mus a 5, were performed\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods and results<\/jats:bold>\n                    The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI\u2010TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451\u00b167\u2009Da. Two Mus a 5 isoforms (p\n                    <jats:italic>I<\/jats:italic>\n                    7.7 and 8.0) were detected by 2D immunoblot with an identical N\u2010terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration\u2010dependent manner\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion<\/jats:bold>\n                    Mus a 5 is a functional allergen and a candidate for the component\u2010resolved allergy diagnosis of banana allergy.\n                  <\/jats:p>","DOI":"10.1002\/mnfr.201100541","type":"journal-article","created":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T03:32:00Z","timestamp":1323401520000},"page":"446-453","source":"Crossref","is-referenced-by-count":23,"title":["Molecular and immunological characterization of Mus a 5 allergen from banana fruit"],"prefix":"10.1002","volume":"56","author":[{"given":"Ivana","family":"Aleksic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milica","family":"Popovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajna","family":"Dimitrijevic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uros","family":"Andjelkovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Vassilopoulou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athanassios","family":"Sinaniotis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Atanaskovic\u2010Markovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buko","family":"Lindner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnd","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos G.","family":"Papadopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Gavrovic\u2010Jankulovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(90)90202-F"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(94)70046-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1996.tb00557.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.02186.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62090-X"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.10.034"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2005.02286.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.1611"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000059400"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00526.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0300935"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2010.03619.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2010.01125.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-6684-0_10"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1994.05020273.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00018553"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20020423"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-8130(00)00109-4"},{"key":"e_1_2_1_20_2","first-page":"82","article-title":"Allergenic proteins of Hevea brasiliensis latex fraction","volume":"10","author":"Sunderasan E.","year":"1995","journal-title":"J. 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