{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:18:43Z","timestamp":1760645923364},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,5,18]],"date-time":"2012-05-18T00:00:00Z","timestamp":1337299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scottish Government Rural and Environment Science and Analytical Services Division"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Cardiovascular disease is the major cause of death in the world. Low dietary folate, elevated homocysteine, and high circulating cholesterol are risk factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We investigated whether folate and\/or <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> vitamin deficiency would change lipoprotein and fatty acid metabolism and lipid accumulation in the aorta adventitia of <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>po<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> null mice. Mice (<jats:italic>n<\/jats:italic> = 10 per group) were fed a control (<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>; 4%) or high saturated fat (<jats:styled-content style=\"fixed-case\">HF<\/jats:styled-content>; 21%), and high cholesterol (0.15%) diet for 16 weeks. Folate (<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\u2010) or folate, <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>6 and <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>12 deficiency (<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010) were imposed on these diets. Feeding a <jats:styled-content style=\"fixed-case\">HF<\/jats:styled-content> diet increased plasma and liver total cholesterol and <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> cholesterol (two\u2010 to threefold; <jats:italic>p<\/jats:italic> &lt; 0.05). Total cholesterol increased (twofold; <jats:italic>p<\/jats:italic> &lt; 0.05) in aorta adventitial lipid in response to <jats:styled-content style=\"fixed-case\">HF<\/jats:styled-content>. Feeding a diet depleted of folate and <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> vitamins (<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010) significantly increased cholesterol accumulation in both liver and aorta adventitial lipid (approximately 50\u201370%; <jats:italic>p<\/jats:italic> &lt; 0.05). Moreover, the proportions of fatty acids in hepatic and adventitial lipid was significantly changed by <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> vitamin depletion, measured as an increase in saturated fatty acids (approximately 15%) and a decrease (approximately 11%) in monounsaturated fatty acids (<jats:italic>p<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>B vitamin deficiency perturbs lipid metabolism in <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>po<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> null mice, causing accumulation of proatherogenic cholesterol and fatty acids in the aorta adventitia.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201100694","type":"journal-article","created":{"date-parts":[[2012,5,18]],"date-time":"2012-05-18T11:07:46Z","timestamp":1337339266000},"page":"1122-1130","source":"Crossref","is-referenced-by-count":11,"title":["Nutritional <scp>B<\/scp> vitamin deficiency disrupts lipid metabolism causing accumulation of proatherogenic lipoproteins in the aorta adventitia of <scp>A<\/scp>po<scp>E<\/scp> null mice"],"prefix":"10.1002","volume":"56","author":[{"given":"Christopher J.","family":"McNeil","sequence":"first","affiliation":[{"name":"Natural Products Group Division of Lifelong Health Rowett Institute of Nutrition and Health University of Aberdeen Aberdeen United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H.","family":"Beattie","sequence":"additional","affiliation":[{"name":"Micronutrients Group Division of Lifelong Health Rowett Institute of Nutrition and Health University of Aberdeen United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret\u2010Jane","family":"Gordon","sequence":"additional","affiliation":[{"name":"Micronutrients Group Division of Lifelong Health Rowett Institute of Nutrition and Health University of Aberdeen United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynn P.","family":"Pirie","sequence":"additional","affiliation":[{"name":"Natural Products Group Division of Lifelong Health Rowett Institute of Nutrition and Health University of Aberdeen Aberdeen United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan J.","family":"Duthie","sequence":"additional","affiliation":[{"name":"Natural Products Group Division of Lifelong Health Rowett Institute of Nutrition and Health University of Aberdeen Aberdeen United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,18]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Global Atlas on Cardiovascular Disease Prevention and Control","author":"Mendis S.","year":"2011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200208000-00007"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1995.03530130055028"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/hq0102.102190"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665108007076"},{"key":"e_1_2_7_7_1","first-page":"1","article-title":"The natural history of homocystinuria due to cystathione\u2010B\u2010synthase deficiency","volume":"37","author":"Mudd S. 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