{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:32:03Z","timestamp":1791232323336,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,8,15]],"date-time":"2012-08-15T00:00:00Z","timestamp":1344988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p>The aqueous extracts of <jats:italic>Hibiscus sabdariffa<\/jats:italic> have been commonly used in folk medicine. Nevertheless, the compounds or metabolites responsible for its healthy effects have not yet been identified. The major metabolites present in rat plasma after acute ingestion of a polyphenol\u2010enriched <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>ibiscus sabdariffa<\/jats:italic> extract were characterized and quantified in order to study their bioavailability. The antioxidant status of the plasma samples was also measured through several complementary antioxidant techniques. High\u2010performance liquid chromatography coupled to time\u2010of\u2010flight mass spectrometry (<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ESI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) was used for the bioavailability study. The antioxidant status was measured by ferric reducing ability of plasma method, thiobarbituric acid reactive substances assay, and superoxide dismutase activity assay. Seventeen polyphenols and metabolites have been detected and quantified. Eleven of these compounds were metabolites. Although phenolic acids were found in plasma without any modification in their structures, most flavonols were found as quercetin or kaempferol glucuronide conjugates. Flavonol glucuronide conjugates, which show longer half\u2010life elimination values, are proposed to contribute to the observed lipid peroxidation inhibitory activity in the cellular membranes. By contrast, phenolic acids appear to exert their antioxidant activity through ferric ion reduction and superoxide scavenging at shorter times. We propose that flavonol\u2010conjugated forms (quercetin and kaempferol) may be the compounds responsible for the observed antioxidant effects and contribute to the healthy effects of <jats:italic><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>. sabdariffa<\/jats:italic> polyphenolic extract.<\/jats:p>","DOI":"10.1002\/mnfr.201200091","type":"journal-article","created":{"date-parts":[[2012,8,15]],"date-time":"2012-08-15T09:15:10Z","timestamp":1345022110000},"page":"1590-1595","source":"Crossref","is-referenced-by-count":62,"title":["Bioavailability study of a polyphenol\u2010enriched extract from <i><scp>H<\/scp>ibiscus sabdariffa<\/i> in rats and associated antioxidant status"],"prefix":"10.1002","volume":"56","author":[{"given":"Salvador","family":"Fern\u00e1ndez\u2010Arroyo","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Sciences University of Granada  Granada Spain"},{"name":"Functional Food Research and Development Center Health Science Technological Park  Armilla (Granada) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Herranz\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Moleculary Celular (IBMC) Universidad Miguel Hern\u00e1ndez  Alicante Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Beltr\u00e1n\u2010Deb\u00f3n","sequence":"additional","affiliation":[{"name":"Centre de Recerca Biom\u00e8dica Hospital Universitari de Sant Joan IISPV Universitat Rovira i Virgili  Reus Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Borr\u00e1s\u2010Linares","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Sciences University of Granada  Granada Spain"},{"name":"Functional Food Research and Development Center Health Science Technological Park  Armilla (Granada) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Barraj\u00f3n\u2010Catal\u00e1n","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Moleculary Celular (IBMC) Universidad Miguel Hern\u00e1ndez  Alicante Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Joven","sequence":"additional","affiliation":[{"name":"Centre de Recerca Biom\u00e8dica Hospital Universitari de Sant Joan IISPV Universitat Rovira i Virgili  Reus Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Fern\u00e1ndez\u2010Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Sciences University of Granada  Granada Spain"},{"name":"Functional Food Research and Development Center Health Science Technological Park  Armilla (Granada) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Segura\u2010Carretero","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Sciences University of Granada  Granada Spain"},{"name":"Functional Food Research and Development Center Health Science Technological Park  Armilla (Granada) Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicente","family":"Micol","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Moleculary Celular (IBMC) Universidad Miguel Hern\u00e1ndez  Alicante Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8,15]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/130.8.2073S"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-961391"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900298"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2011.03.040"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200500084"},{"key":"e_1_2_4_7_1","first-page":"276","article-title":"Antioxidant and antihyperlipidemic activity of \n                     Hibiscus sabdariffa Linn. leaves and calyces extracts in rats","volume":"47","author":"Ochani P. 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