{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T01:11:12Z","timestamp":1789780272799,"version":"4.0.1"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,12,2]],"date-time":"2012-12-02T00:00:00Z","timestamp":1354406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Whole genome transcriptome analysis of male and female <jats:italic>beta\u2010carotene 15,15\u2032\u2010monooxygenase<\/jats:italic> knockout (<jats:italic>Bcmo1<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic>) and <jats:italic>Bcmo1<jats:sup>+\/+<\/jats:sup><\/jats:italic> (wild\u2010type) mice with or without 14 wk of BC supplementation was done. We previously showed that only 1.8% of the genes regulated by BC in lung were also regulated in liver and inguinal white adipose tissue (iWAT), suggesting lung specific responses. Here, we explicitly questioned the lung specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We show that BC supplementation resulted in an opposite direction of gene\u2010regulation in male compared to female <jats:italic>Bcmo1<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> mice in lung, liver, and iWAT. This supports a systemic effect of BC on steroid hormone metabolism mediated responses. Lung, liver, and iWAT of female <jats:italic>Bcmo1<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> mice showed an increased inflammatory response, which was counteracted by supplementation of BC. This supports a genotype dependent increased sensitivity of female mice for vitamin A deficiency. Finally, the effect of BC on <jats:italic>Wnt<\/jats:italic> signaling in male <jats:italic>Bcmo1<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> mice was examined. <jats:italic>Frizzled homolog 6<\/jats:italic> (<jats:italic>Fzd6<\/jats:italic>) downregulation was seen in all three tissues. <jats:italic>Collagen triple helix containing 1<\/jats:italic> (<jats:italic>Cthrc1<\/jats:italic>) downregulation was seen in lung tissue only, suggesting specificity. Upregulation of genes involved in oxygen sensing was seen in lung and iWAT, while protocadherin upregulation was only seen in lung.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results demonstrate that effects of BC are strongly sex dependent. While effects of BC on hormone metabolism mediated responses and inflammation are systemic, effects on <jats:italic>Wnt<\/jats:italic> signaling may be lung specific.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201200277","type":"journal-article","created":{"date-parts":[[2012,12,2]],"date-time":"2012-12-02T18:22:17Z","timestamp":1354472537000},"page":"307-319","source":"Crossref","is-referenced-by-count":5,"title":["Organ specificity of beta\u2010carotene induced lung gene\u2010expression changes in <i>Bcmo1<sup>\u2212\/\u2212<\/sup><\/i> mice"],"prefix":"10.1002","volume":"57","author":[{"given":"Yvonne G. J.","family":"van Helden","sequence":"first","affiliation":[{"name":"Human and Animal Physiology Wageningen University  Wageningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger W. 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