{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:25:15Z","timestamp":1791966315578,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T00:00:00Z","timestamp":1347408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FPU fellowship from the Ministerio de Educaci\u00f3n"},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"crossref","award":["AGR-4783"],"award-info":[{"award-number":["AGR-4783"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"crossref","award":["P09AGR-4783"],"award-info":[{"award-number":["P09AGR-4783"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"crossref","award":["AGL2010-19643-C02-02"],"award-info":[{"award-number":["AGL2010-19643-C02-02"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"crossref","award":["TRA2009_0047"],"award-info":[{"award-number":["TRA2009_0047"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>The only treatment available for coeliac disease (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>) is a strict diet in which the intake of wheat, barley, rye, or oats is avoided. Barley is a major cereal crop, grown mainly for its use in brewing, and it has high nutritional value. The identification of varieties with a reduced toxicity profile may contribute to improve the diet, the quality of life and health of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Searching for harmless barleys, we investigated accessions of malting and wild barley, used for developing new cultivated cereals. The <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> toxicity profile of barleys was screened using G12 antibody and cell proliferation and <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 release from peripheral blood mononuclear cells and intestinal biopsies from <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> patients. We found a direct correlation between the reactivity with <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>12 and the immunogenicity of the different barleys.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The malting barleys were less immunogenic, with reduced levels of toxic gluten, and were possibly less harmful to <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> patients. Our findings could raise the prospect of breeding barley species with low levels of harmful gluten, and the attractive goal of developing nontoxic barley cultivars, always taking into account the <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>odex standard for foods for special dietary use for persons intolerant to gluten.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201200358","type":"journal-article","created":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T07:22:08Z","timestamp":1347434528000},"page":"1697-1707","source":"Crossref","is-referenced-by-count":32,"title":["Significant differences in coeliac immunotoxicity of barley varieties"],"prefix":"10.1002","volume":"56","author":[{"given":"Isabel","family":"Comino","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Farmacia Universidad de Sevilla Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Real","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Farmacia Universidad de Sevilla Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Gil\u2010Humanes","sequence":"additional","affiliation":[{"name":"Instituto de Agricultura Sostenible (C.S.I.C.)  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Pist\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Agricultura Sostenible (C.S.I.C.)  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"de Lorenzo","sequence":"additional","affiliation":[{"name":"Centro Nacional de Biotecnolog\u00eda (C.N.B.\u2010C.S.I.C.) Departamento de Gen\u00e9tica Molecular de Plantas Campus Universidad Aut\u00f3noma  Cantoblanco Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma de Lourdes","family":"Moreno","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Farmacia Universidad de Sevilla Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel \u00c1ngel","family":"L\u00f3pez\u2010Casado","sequence":"additional","affiliation":[{"name":"Hospital Virgen de las Nieves  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Lorite","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental Campus Universitario Las Lagunillas  Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngel","family":"Cebolla","sequence":"additional","affiliation":[{"name":"Biomedal S.L. Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma Isabel","family":"Torres","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Experimental Campus Universitario Las Lagunillas  Ja\u00e9n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Barro","sequence":"additional","affiliation":[{"name":"Instituto de Agricultura Sostenible (C.S.I.C.)  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Sousa","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Farmacia Universidad de Sevilla Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcs.2008.02.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2008.12.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri885"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0010001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.225268"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.02.015"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.1.345"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.1.187"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2011.02376.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)91819-P"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306885101"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1998.00397.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.33606"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074129"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001012"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114509371767"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906773106"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1007773107"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017366"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejim.2011.08.030"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejim.2011.09.016"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcr049"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.110.082537"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.128967"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/87.2.405"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002294"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006313"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.502"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.026708"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.05.028"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1998.tb03066.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2009.03.013"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0950-3528(95)90032-2"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI106108"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/jcrs.1998.0235"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-5223.1992.tb00227.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2008.06.008"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-011-9446-4"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-003-1548-x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-005-2046-0"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1139\/g93-055"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.06.017"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.11.003"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2004.12.005"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.030551"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.43.2.190"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)91837-6"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2010.04452.x"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/02652030600740637"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.53"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.50.5.624"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.201200358","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.201200358","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201200358","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T11:48:09Z","timestamp":1697456889000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.201200358"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,12]]},"references-count":52,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1002\/mnfr.201200358"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.201200358","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,12]]}}}