{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:46:53Z","timestamp":1791193613377,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,10,12]],"date-time":"2012-10-12T00:00:00Z","timestamp":1350000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Cow's milk allergy is the most prevalent food allergy in infants whose immune system development is critically stimulated during postnatal gut colonization by commensal bacteria. Allergenic potential of cow's milk \u03b2\u2010lactoglobulin (<jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content>) and caseins (<jats:styled-content style=\"fixed-case\">CAS<\/jats:styled-content>) was investigated in germ\u2010free (<jats:styled-content style=\"fixed-case\">GF<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice and in <jats:styled-content style=\"fixed-case\">GF<\/jats:styled-content> mice conventionalized (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>d) at 6 weeks of age.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Oral sensitization to cow's milk in the presence of cholera toxin led to higher <jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content>\u2010specific <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>1, and <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>2a responses in <jats:styled-content style=\"fixed-case\">GF<\/jats:styled-content> mice than in conventional (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>) mice. No significant difference was observed for <jats:styled-content style=\"fixed-case\">CAS<\/jats:styled-content>\u2010specific <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> responses although <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>1 responses to \u03b1<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>1\u2010 and \u03ba\u2010caseins were higher in <jats:styled-content style=\"fixed-case\">GF<\/jats:styled-content> mice than in <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> mice. <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>d mice, orally inoculated with fecal preparations from <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> mice, also displayed biased antibody responses compared to <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> mice. Secretion of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>h2 cytokines by <jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content>\u2010 and <jats:styled-content style=\"fixed-case\">CAS<\/jats:styled-content>\u2010reactivated splenocytes of <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>d mice was similar to that of <jats:styled-content style=\"fixed-case\">GF<\/jats:styled-content> mice whereas cytokine production by reactivated cells from mesenteric lymph nodes of <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>d mice was equivalent to that of <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Oral sensitization to <jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CAS<\/jats:styled-content> was differentially affected by the absence of gut microbiota and delayed bacterial colonization altered persistently the host immune response to oral sensitization against food antigens.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201200412","type":"journal-article","created":{"date-parts":[[2012,10,12]],"date-time":"2012-10-12T11:51:45Z","timestamp":1350042705000},"page":"1838-1847","source":"Crossref","is-referenced-by-count":19,"title":["Delayed bacterial colonization of the gut alters the host immune response to oral sensitization against cow's milk proteins"],"prefix":"10.1002","volume":"56","author":[{"given":"St\u00e9phanie","family":"Morin","sequence":"first","affiliation":[{"name":"INRA, UR 496 \u2013 Immuno\u2010Allergie Alimentaire CEA\/iBiTeC\u2010S\/SPI  CEA de Saclay Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romy","family":"Fischer","sequence":"additional","affiliation":[{"name":"INRA, UR 496 \u2013 Immuno\u2010Allergie Alimentaire CEA\/iBiTeC\u2010S\/SPI  CEA de Saclay 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