{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:33:44Z","timestamp":1761708824433},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T00:00:00Z","timestamp":1353369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>This study addresses the effects of branched\u2010chain amino acids (BCAA) on global gene expression in liver and skeletal muscle and the molecular mechanisms underlying the improvement in liver cirrhosis using DNA microarray analysis combined with RNase protection assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Male Wistar rats administered carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) repeatedly for 19 weeks as a decompensated cirrhosis model were thereafter given BCAA\u2010enriched diet (AL) or normal diet (LC) for 5 weeks. The control\u2010diet rats without CCl<jats:sub>4<\/jats:sub> administration were used as a normal control group. Gene expression in AL was reversed by twofold greater than in LC in the microarray were selected to elucidate the improvements in nutritional and metabolic disorders. Downregulation of fatty acid translocase (<jats:italic>FAT<\/jats:italic>)<jats:italic>\/Cd36<\/jats:italic>, glutamine synthetase, and pyruvate dehydrogenase kinase isoenzyme 4 is believed to promote lower uptake of fatty acids, lower ammonia incorporation, and higher uptake of glucose, and thus to provide an energy source without using BCAA. Ultimately, the catabolism of BCAA and skeletal muscle protein would be slowed, maintaining BCAA concentrations in blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We established, for the first time, the regulatory gene pathways of processes involved in hepatic fibrosis and energy metabolism (hypoalbuminemia, hyperammonemia, and carbohydrate catabolism, and their relationships) under BCAA supplementation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201200538","type":"journal-article","created":{"date-parts":[[2012,11,20]],"date-time":"2012-11-20T07:26:22Z","timestamp":1353396382000},"page":"291-306","source":"Crossref","is-referenced-by-count":14,"title":["DNA microarray analysis identified molecular pathways mediating the effects of supplementation of branched\u2010chain amino acids on CCl<sub>4<\/sub>\u2010induced cirrhosis in rats"],"prefix":"10.1002","volume":"57","author":[{"given":"Huijuan","family":"Jia","sequence":"first","affiliation":[{"name":"Corporate Sponsored Research Program \u2018Food for Life\u2019 Organization for Interdisciplinary Research Projects The University of Tokyo Bunkyo\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Chemistry Graduate School of Agricultural and Life Sciences The University of Tokyo Bunkyo\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Saito","sequence":"additional","affiliation":[{"name":"Corporate Sponsored Research Program \u2018Food for Life\u2019 Organization for Interdisciplinary Research Projects The University of Tokyo Bunkyo\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisanori","family":"Kato","sequence":"additional","affiliation":[{"name":"Corporate Sponsored Research Program \u2018Food for Life\u2019 Organization for Interdisciplinary Research Projects The University of Tokyo Bunkyo\u2010ku Tokyo Japan"},{"name":"Department of Applied Biological Chemistry Graduate School of Agricultural and Life Sciences The University of Tokyo Bunkyo\u2010ku Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2010.06306.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2008.12.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2009.06.027"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2008.00435.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23248"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-007-2122-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i33.4486"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.21758"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2008.00319.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2008.00434.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018831302578"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hepres.2004.11.009"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2007.00081.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1710751"},{"key":"e_1_2_7_16_1","first-page":"204","article-title":"Overweight and obesity increase the risk for liver cancer in patients with liver cirrhosis and long\u2010term oral supplementation with branched\u2010chain amino acid granules inhibits liver carcinogenesis in heavier patients with liver cirrhosis","volume":"35","author":"Muto Y.","year":"2006","journal-title":"Hepatol. 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