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Here, we aimed to identify compounds capable to render sequential epitopes in egg.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Glutathione was used for in vitro reduction of <jats:styled-content style=\"fixed-case\">OVO<\/jats:styled-content> and circular dichroism analyses were performed. Glutathione reduced <jats:styled-content style=\"fixed-case\">OVO<\/jats:styled-content> in a concentration\u2010dependent manner. Egg white was analyzed for reduced proteins with a thiol probe and by <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>. In unprocessed total egg white, several reduced proteins were detected by the thiol probe, among them reduced ovalbumin could be confirmed with <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> analyses.<\/jats:p><jats:p>Egg\u2010allergics or sensitized controls were tested serologically (<jats:italic>n<\/jats:italic> = 19) for <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> against native and reduced <jats:styled-content style=\"fixed-case\">OVO<\/jats:styled-content> and in skin prick tests (<jats:italic>n<\/jats:italic> = 9). More patients had <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> against reduced than native <jats:styled-content style=\"fixed-case\">OVO<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blots. In skin prick test, five out of seven persistent egg\u2010allergics and none of the controls reacted with reduced <jats:styled-content style=\"fixed-case\">OVO<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Reduced egg proteins are present in natural egg white. Glutathione, which is present in egg and furthermore is used as texture\u2010improving additive in processed food, is capable of reducing <jats:styled-content style=\"fixed-case\">OVO<\/jats:styled-content>. Patients with persistent egg allergy reacted rather to reduce the native <jats:styled-content style=\"fixed-case\">OVO<\/jats:styled-content>. Hence, our data indicate that reduction is a novel natural and processing\u2010associated principle, which contributes to the allergenicity of food.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201200612","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T16:40:48Z","timestamp":1359045648000},"page":"536-544","source":"Crossref","is-referenced-by-count":9,"title":["Glutathione exposes sequential <scp>I<\/scp>g<scp>E<\/scp>\u2010epitopes in ovomucoid relevant in persistent egg allergy"],"prefix":"10.1002","volume":"57","author":[{"given":"Franziska","family":"Roth\u2010Walter","sequence":"first","affiliation":[{"name":"Comparative Medicine, Messerli Research Institute University of Veterinary Medicine Vienna, Vienna Medical University of Vienna and University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"Starkl","sequence":"additional","affiliation":[{"name":"Comparative Immunology and Oncology, Department of Pathophysiology and Allergy Research Centre of Pathophysiology Infectiology and Immunology Medical University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Torsten","family":"Zuberbier","sequence":"additional","affiliation":[{"name":"Clinic for Dermatology Venereology and Allergology Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"Hummel","sequence":"additional","affiliation":[{"name":"VetCore Facility for Research VetOmics University of Veterinary Medicine Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karin","family":"N\u00f6bauer","sequence":"additional","affiliation":[{"name":"VetCore Facility for Research VetOmics University of Veterinary Medicine Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebrahim","family":"Razzazi\u2010Fazeli","sequence":"additional","affiliation":[{"name":"VetCore Facility for Research VetOmics University of Veterinary Medicine Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Brunner","sequence":"additional","affiliation":[{"name":"Comparative Immunology and Oncology, Department of Pathophysiology and Allergy Research Centre of Pathophysiology Infectiology and Immunology Medical University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella","family":"Pali\u2010Sch\u00f6ll","sequence":"additional","affiliation":[{"name":"Comparative Medicine, Messerli Research Institute University of Veterinary Medicine Vienna, Vienna Medical University of Vienna and University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janis","family":"Kinkel","sequence":"additional","affiliation":[{"name":"Comparative Medicine, Messerli Research Institute University of Veterinary Medicine Vienna, Vienna Medical University of Vienna and University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferdinand","family":"Felix","sequence":"additional","affiliation":[{"name":"Comparative Medicine, Messerli Research Institute University of Veterinary Medicine Vienna, Vienna Medical University of Vienna and University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Jensen\u2010Jarolim","sequence":"additional","affiliation":[{"name":"Comparative Medicine, Messerli Research Institute University of Veterinary Medicine Vienna, Vienna Medical University of Vienna and University of Vienna  Austria"},{"name":"Comparative Immunology and Oncology, Department of Pathophysiology and Allergy Research Centre of Pathophysiology Infectiology and Immunology Medical University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamar","family":"Kinaciyan","sequence":"additional","affiliation":[{"name":"Department of Dermatology Division of Immunology Allergy &amp; 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