{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:53:48Z","timestamp":1788872028819,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T00:00:00Z","timestamp":1370822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for Promotion of Science","doi-asserted-by":"crossref","award":["23580187"],"award-info":[{"award-number":["23580187"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for Promotion of Science","doi-asserted-by":"crossref","award":["22248014"],"award-info":[{"award-number":["22248014"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2013,10]]},"abstract":"\n Scope<\/jats:title>\n \n 10\u2010Hydroxy\u20102\u2010decenoic acid (10\n H<\/jats:styled-content>\n 2\n DA<\/jats:styled-content>\n ) is one of the unique medium\u2010chain fatty acids (\n MCFA<\/jats:styled-content>\n s) specifically found in royal jelly. We hypothesize that 10\n H<\/jats:styled-content>\n 2\n DA<\/jats:styled-content>\n has multiple biological functions and may aid in 5\u2032\u2010\n AMP<\/jats:styled-content>\n \u2010activated protein kinase (\n AMPK<\/jats:styled-content>\n ) activation and affect the glucose transport system in skeletal muscle.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and results<\/jats:title>\n \n We examined whether various\n MCFA<\/jats:styled-content>\n s present in royal jelly activated\n AMPK<\/jats:styled-content>\n \u03b1. Treatment of\n L<\/jats:styled-content>\n 6 myotubes with various\n MCFA<\/jats:styled-content>\n s showed that 10\n H<\/jats:styled-content>\n 2\n DA<\/jats:styled-content>\n administration resulted in a significant increase in phosphorylated\n AMPK<\/jats:styled-content>\n \u03b1. 10\n H<\/jats:styled-content>\n 2\n DA<\/jats:styled-content>\n activates\n AMPK<\/jats:styled-content>\n independently of insulin and significantly increased glucose uptake into\n L<\/jats:styled-content>\n 6 myotubes following translocation of glucose transporter 4 (\n G<\/jats:styled-content>\n lut4) to the plasma membrane (\n PM<\/jats:styled-content>\n ). The activation was induced by the upstream kinase\n C<\/jats:styled-content>\n a\n 2+<\/jats:sup>\n \/calmodulin\u2010dependent kinase kinase \u03b2, but was independent of changes in\n AMP<\/jats:styled-content>\n :\n ATP<\/jats:styled-content>\n ratio and the liver kinase B1 pathway. Oral administration of 10\n H<\/jats:styled-content>\n 2\n DA<\/jats:styled-content>\n significantly stimulated phosphorylation of\n AMPK<\/jats:styled-content>\n and Glut4 translocation to the PM in mouse skeletal muscle.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n These findings indicate that (i) 10\n H<\/jats:styled-content>\n 2\n DA<\/jats:styled-content>\n activates\n AMPK<\/jats:styled-content>\n , and insulin independently enhances glucose uptake following translocation of\n G<\/jats:styled-content>\n lut4 to\n PM<\/jats:styled-content>\n , (ii) activation of\n AMPK<\/jats:styled-content>\n \u03b1 by 10\n H<\/jats:styled-content>\n 2\n DA<\/jats:styled-content>\n is mediated via extracellular\n C<\/jats:styled-content>\n a\n 2+<\/jats:sup>\n \u2010dependent\n C<\/jats:styled-content>\n a\n 2+<\/jats:sup>\n \/calmodulin\u2010dependent kinase kinase \u03b2, without alteration in the\n AMP<\/jats:styled-content>\n :\n ATP<\/jats:styled-content>\n ratio, and liver kinase B1 was not involved in the activation.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/mnfr.201300041","type":"journal-article","created":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T03:03:39Z","timestamp":1370833419000},"page":"1794-1802","source":"Crossref","is-referenced-by-count":45,"title":["10\u2010Hydroxy\u20102\u2010decenoic acid, a unique medium\u2010chain fatty acid, activates 5'\u2010\n AMP<\/scp>\n \u2010activated protein kinase in\n L<\/scp>\n 6 myotubes and mice"],"prefix":"10.1002","volume":"57","author":[{"given":"Masathito","family":"Takikawa","sequence":"first","affiliation":[{"name":"College of Bioscience and Biotechnology Chubu University, Kasugai Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Kumagai","sequence":"additional","affiliation":[{"name":"College of Bioscience and Biotechnology Chubu University, Kasugai Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harumi","family":"Hirata","sequence":"additional","affiliation":[{"name":"College of Bioscience and Biotechnology Chubu University, Kasugai Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Soga","sequence":"additional","affiliation":[{"name":"College of Bioscience and Biotechnology Chubu University, Kasugai Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science Kobe University, Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Ueda","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science Kobe University, Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Ashida","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural Science Kobe University, Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takanori","family":"Tsuda","sequence":"additional","affiliation":[{"name":"College of Bioscience and Biotechnology Chubu University, Kasugai Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199907223410406"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.44.27879"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.11.018"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2004.12.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI13505"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202489200"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1997.273.6.E1107"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3945\/jn.109.118216"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/db09-0482"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/186633a0"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/1831270a0"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.53.331"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2008.0289"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.31.2103"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1248\/yakushi.127.1877"},{"key":"e_1_2_7_17_1","first-page":"19","article-title":"Royal jelly and its efficacy","volume":"11","author":"Lakin A.","year":"1993","journal-title":"Int. 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