{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:52:31Z","timestamp":1772999551139,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Austrian Science Fund","award":["P-22559-B11"],"award-info":[{"award-number":["P-22559-B11"]}]},{"name":"Austrian Science Fund","award":["SFB-F4608"],"award-info":[{"award-number":["SFB-F4608"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Birch pollen associated allergy to mung bean sprouts is caused by cross\u2010reactivity between the birch pollen allergen <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>et v 1 and the mung bean allergen <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 1. We aimed to determine the allergenicity of the cytokinin\u2010specific binding protein from mung bean (<jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 6), another allergen related to <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>et v 1 with only 31% sequence identity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Bet v 1, <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ly m 4, <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 1, and <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 6 were produced in <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>scherichia coli<\/jats:italic>. In an <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, 73 and 32% of <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>et v 1\u2010sensitized birch\u2010allergic patients\u2019 sera (<jats:italic>n<\/jats:italic> = 60) showed <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> binding to <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 1 and <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 6, respectively. Of 19 patients who reported allergic reactions or had positive prick\u2010to\u2010prick tests to mung bean sprouts, 79% showed <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> binding to <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 1 and 63% showed <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> binding to <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 6. <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>et v 1 completely inhibited <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> binding to both mung bean allergens. <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 6 showed partial cross\u2010reactivity with <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 1 and activated basophils sensitized with mung bean allergic patients\u2019 sera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We demonstrated <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> cross\u2010reactivity despite low sequence identity between <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 6 and other <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>et v 1\u2010related allergens. Thus, <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> binding to <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ig r 6 may contribute to birch pollinosis\u2010associated mung bean sprout allergy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201300153","type":"journal-article","created":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T15:15:08Z","timestamp":1378048508000},"page":"625-634","source":"Crossref","is-referenced-by-count":30,"title":["<scp>V<\/scp>ig r 6, the cytokinin\u2010specific binding protein from mung bean (<i><scp>V<\/scp>igna radiata<\/i>) sprouts, cross\u2010reacts with <scp>B<\/scp>et v 1\u2010related allergens and binds <scp>I<\/scp>g<scp>E<\/scp> from birch pollen allergic patients\u2019 sera"],"prefix":"10.1002","volume":"58","author":[{"given":"Eva Elisabeth","family":"Guhsl","sequence":"first","affiliation":[{"name":"Department of Pathophysiology and Allergy Research, Medical University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerlinde","family":"Hofstetter","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology and Allergy Research, Medical University of Vienna  Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Hemmer","sequence":"additional","affiliation":[{"name":"Floridsdorfer Allergiezentrum  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christof","family":"Ebner","sequence":"additional","affiliation":[{"name":"Ambulatorium f\u00fcr Allergie und klinische Immunologie  Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Vieths","sequence":"additional","affiliation":[{"name":"Paul\u2010Ehrlich\u2010Institut  Langen 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