{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T18:34:43Z","timestamp":1764873283923,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,9,17]],"date-time":"2013-09-17T00:00:00Z","timestamp":1379376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Iijima Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Recently, we found that dipeptide <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rg\u2010<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>he (<jats:styled-content style=\"fixed-case\">RF<\/jats:styled-content>) had cholecystokinin (<jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>)\u2010dependent vasorelaxing activity. The <jats:styled-content style=\"fixed-case\">RF<\/jats:styled-content> sequence is often observed in the primary structure of natural food proteins. In the current study, we investigated enzymatic conditions for the release of <jats:styled-content style=\"fixed-case\">RF<\/jats:styled-content>\u2010related peptides from rice glutelin, a major storage protein, using gastrointestinal proteases. <jats:styled-content style=\"fixed-case\">RF<\/jats:styled-content>\u2010related peptides were then characterized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>It was found that <jats:styled-content style=\"fixed-case\">RF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>le\u2010<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>is\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rg\u2010<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>he (<jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content>) were released in the chymotrypsin digest of the partial structure of rice glutelin. We then focused on previously unidentified <jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content>, corresponding to rice glutelin(155\u2212158). <jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content> had vasorelaxing activity in the mesenteric artery of spontaneous hypertensive rats (<jats:styled-content style=\"fixed-case\">SHR<\/jats:styled-content>s). Orally administered <jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content> lowered systolic blood pressure in <jats:styled-content style=\"fixed-case\">SHR<\/jats:styled-content>s. The antihypertensive activity of <jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content> was more potent and long\u2010lasting than that of <jats:styled-content style=\"fixed-case\">RF<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content>\u2010induced vasorelaxing activity was not blocked by inhibitors of nitric oxide synthase and cyclooxygenase, but by an antagonist for <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content><jats:sub>1<\/jats:sub> receptor. <jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content> also had <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u2010like suppressive activities in food intake and gastrointestinal transit. <jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content> increased intracellular <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>a<jats:sup>2+<\/jats:sup> flux and <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content> release in the enteroendocrine cell <jats:styled-content style=\"fixed-case\">STC<\/jats:styled-content>\u20101.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IHRF<\/jats:styled-content>, a novel <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u2010dependent vasorelaxing peptide, decreases both blood pressure and food intake in rodents.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201300334","type":"journal-article","created":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T14:11:12Z","timestamp":1381241472000},"page":"359-364","source":"Crossref","is-referenced-by-count":35,"title":["Characterization of <scp>I<\/scp>le\u2010<scp>H<\/scp>is\u2010<scp>A<\/scp>rg\u2010<scp>P<\/scp>he, a novel rice\u2010derived vasorelaxing peptide with hypotensive and anorexigenic 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