{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:55:05Z","timestamp":1790740505617,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,5,15]],"date-time":"2014-05-15T00:00:00Z","timestamp":1400112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2010\/01410-0"],"award-info":[{"award-number":["2010\/01410-0"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2012\/10872-2"],"award-info":[{"award-number":["2012\/10872-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>A compromised nutritional status in methyl\u2010group donors may provoke several molecular alterations triggering the development of nonalcoholic fatty liver disease (NAFLD) in humans and experimental animals. In this study, we investigated a role and the underlying molecular mechanisms of methionine metabolic pathway malfunctions in the pathogenesis of NAFLD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We fed female Swiss albino mice a control (methionine\u2010adequate) diet and two experimental (methionine\u2010deficient or methionine\u2010supplemented) diets for 10 weeks, and the levels of one\u2010carbon metabolites, expression of one\u2010carbon and lipid metabolism genes in the livers were evaluated. We demonstrate that both experimental diets increased hepatic levels of <jats:italic>S<\/jats:italic>\u2010adenosyl\u2010<jats:sc>l<\/jats:sc>\u2010homocysteine and homocysteine, altered expression of one\u2010carbon and lipid metabolism genes, and caused lipid accumulation, especially in mice fed the methionine\u2010deficient diet. Markers of oxidative and ER stress response were also elevated in the livers of mice fed either diet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicate that both dietary methionine deficiency and methionine supplementation can induce molecular abnormalities in the liver associated with the development of NAFLD, including deregulation in lipid and one\u2010carbon metabolic pathways, and induction of oxidative and ER stress. These pathophysiological events may ultimately lead to lipid accumulation in the livers, triggering the development of NAFLD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201300726","type":"journal-article","created":{"date-parts":[[2014,5,15]],"date-time":"2014-05-15T09:47:30Z","timestamp":1400147250000},"page":"1502-1512","source":"Crossref","is-referenced-by-count":62,"title":["Effect of methionine\u2010deficient and methionine\u2010supplemented diets on the hepatic one\u2010carbon and lipid metabolism in mice"],"prefix":"10.1002","volume":"58","author":[{"given":"Alexandre Ferro","family":"Aissa","sequence":"first","affiliation":[{"name":"Department of Genetic Faculty of Medicine of Ribeir\u00e3o Preto, University of S\u00e3o Paulo  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volodymyr","family":"Tryndyak","sequence":"additional","affiliation":[{"name":"Division of Biochemical Toxicology National Center for Toxicological Research, U.S. Food and Drug Administration  Jefferson AR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"de Conti","sequence":"additional","affiliation":[{"name":"Division of Biochemical Toxicology National Center for Toxicological Research, U.S. Food and Drug Administration  Jefferson AR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stepan","family":"Melnyk","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Arkansas for Medical Sciences  Little Rock AR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarsila Daysy Ursula Hermogenes","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis Toxicology and Food Science, Faculty of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lourdes Pires","family":"Bianchi","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis Toxicology and Food Science, Faculty of Pharmaceutical Sciences of Ribeir\u00e3o Preto, University of S\u00e3o Paulo  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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