{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:03:16Z","timestamp":1791201796738,"version":"4.2.0"},"reference-count":63,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,6,26]],"date-time":"2015-06-26T00:00:00Z","timestamp":1435276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002715","name":"Alberta Livestock and Meat Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002715","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Egg ovotransferrin\u2010derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide\u2010mediated vasorelaxation. The main objective of the present study was to investigate the molecular mechanism of this peptide through transcriptome analysis by RNAseq technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Total RNA was extracted from kidney and mesenteric arteries; the RNAseq libraries (from untreated and IRW\u2010treated groups) were constructed and subjected to sequence using HiSeq 2000 system (Illumina) system. A total of 12\u00a0764 and 13\u00a0352 genes were detected in kidney and mesenteric arteries, respectively. The differentially expressed (DE) genes between untreated and IRW\u2010treated groups were identified and the functional analysis through ingenuity pathway analysis revealed a greater role of DE genes identified from mesenteric arteries than that of kidney in modulating various cardiovascular functions. Subsequent qPCR analysis further confirmed that IRW significantly increased the expression of ACE\u20102, ABCB\u20101, IRF\u20108, and CDH\u20101 while significantly decreased the expression ICAM\u20101 and VCAM\u20101 in mesenteric arteries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our research showed for the first time that ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 and decreased proinflammatory genes expression.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201500050","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T15:28:22Z","timestamp":1432740502000},"page":"1735-1744","source":"Crossref","is-referenced-by-count":85,"title":["Egg ovotransferrin\u2010derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats"],"prefix":"10.1002","volume":"59","author":[{"given":"Kaustav","family":"Majumder","sequence":"first","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science Faculty of Agricultural, Life and Environmental Sciences, University of Alberta  Edmonton AB Canada"},{"name":"Cardiovascular Research Centre, University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanxiang","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science Faculty of Agricultural, Life and Environmental Sciences, University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science Faculty of Agricultural, Life and Environmental Sciences, University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LeLuo","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science Faculty of Agricultural, Life and Environmental Sciences, University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra T.","family":"Davidge","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Centre, University of Alberta  Edmonton AB Canada"},{"name":"Women and Children's Health Research Institute, University of Alberta  Edmonton AB Canada"},{"name":"Department of Obstetrics and Gynecology Faculty of Medicine and Dentistry University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science Faculty of Agricultural, Life and Environmental Sciences, University of Alberta  Edmonton AB Canada"},{"name":"Cardiovascular Research Centre, University of Alberta  Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","unstructured":"WHO (World Health Organization). A Global Brief on Hypertension silent killer global public health crisis\u2010 2013. Available from: URL:http:\/\/apps.who.int\/iris\/bitstream\/10665\/79059\/1\/WHO_DCO_WHD_2013.2eng.pdf?ua=1.","DOI":"10.5005\/ijopmr-24-1-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c110058"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0828-282X(08)70617-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.11.3_Pt_2.II37"},{"key":"e_1_2_8_6_1","first-page":"1471","article-title":"Diagnosis of secondary hypertension: an age\u2010based approach","volume":"82","author":"Viera A. J.","year":"2010","journal-title":"Am. Fam. Physician"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhh.2010.85"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2010.11.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/07853890.2012.663926"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2891-9-52"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.08.005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0b013e3283333813"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2011.08.010"},{"key":"e_1_2_8_14_1","first-page":"247","volume-title":"Bioactive Proteins and Peptides from Egg Proteins","author":"Wu J.","year":"2010"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.6.1457"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2010.04.027"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.05.093"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf3046076"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082829"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2012.2541"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114514001147"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf404616f"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2009.08.005"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf405189y"},{"key":"e_1_2_8_25_1","first-page":"107","volume-title":"Chapter 5\u2014Emerging RNA\u2010Seq Applications in Food Science\u2014Comprehensive Analytical Chemistry","author":"Vald\u00e9s A.","year":"2014"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2484"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3001"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2013-14-4-r36"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092592"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-003-0039-5"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-396485-4.00026-8"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.08.023"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00919.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200606001-00008"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(98)00051-5"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2009.05.005"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2fo10192k"},{"key":"e_1_2_8_40_1","first-page":"313","article-title":"Peptides with antihypertensive activity obtained from milk and egg proteins","volume":"23","author":"Aleixandre A.","year":"2008","journal-title":"Nutr. Hosp."},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf9018245"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.5604\/17322693.1043606"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2012.02.010"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.06.034"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400736"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/202759"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728221003709784"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2010.06.003"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00059-007-3073-1"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathol.4.110807.092155"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2012.11.002"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200443090-00001"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2009.02.019"},{"key":"e_1_2_8_54_1","first-page":"17","article-title":"Reverse cholesterol transport and atherosclerosis. A mini review","volume":"45","author":"Cucuianu M.","year":"2007","journal-title":"Rom. J. Intern. Med."},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.08.022"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1089\/gtmb.2014.0065"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2009.03440.x"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MIB.0000209791.98866.ba"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(95)80212-5"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200601018"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000141146.95728.da"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01070-13"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.278101"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2007.05.007"}],"container-title":["Molecular Nutrition &amp; Food Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmnfr.201500050","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201500050","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mnfr.201500050","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mnfr.201500050","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T11:01:10Z","timestamp":1696330870000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mnfr.201500050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,26]]},"references-count":63,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1002\/mnfr.201500050"],"URL":"https:\/\/doi.org\/10.1002\/mnfr.201500050","archive":["Portico"],"relation":{},"ISSN":["1613-4125","1613-4133"],"issn-type":[{"value":"1613-4125","type":"print"},{"value":"1613-4133","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,26]]}}}