{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:50:56Z","timestamp":1786978256431,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,4,21]],"date-time":"2016-04-21T00:00:00Z","timestamp":1461196800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81102130"],"award-info":[{"award-number":["81102130"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec>\n                    <jats:title>Scope<\/jats:title>\n                    <jats:p>\n                      We and others recently showed that soyasaponin Bb (SS\n                      <jats:sub>Bb<\/jats:sub>\n                      ) inhibited lipopolysaccharide (LPS)\u2010induced inflammation in macrophages. Since the recruitment of toll\u2010like receptor 4 (TLR4) into lipid rafts is vital for LPS\u2010initiated signaling, we investigated whether this process would be modulated by SS\n                      <jats:sub>Bb<\/jats:sub>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      By using sucrose gradient ultracentrifuge, we found that pretreatment of macrophages with SS\n                      <jats:sub>Bb<\/jats:sub>\n                      inhibited LPS\u2010induced recruitments of TLR4, myeloid differentiation primary response protein 88 (MyD88) and Toll\/IL\u20101 receptor domain\u2010containing adaptor inducing interferon\u2010\u03b2 (TRIF) into fractions enriched with lipid rafts marker flotillin\u20101. We also found SS\n                      <jats:sub>Bb<\/jats:sub>\n                      decreased co\u2010localization of TLR4 and lipid rafts by utilizing confocal immunofluorescence microscopy. Additionally, we observed that SS\n                      <jats:sub>Bb<\/jats:sub>\n                      suppressed LPS\u2010induced formation of TLR4\/MyD88 and TLR4\/TRIF complexes, production of pro\u2010inflammatory molecules, and activation of nuclear factor kappa B (NF\u2010\u03baB). Furthermore, we found that these inhibitory effects of SS\n                      <jats:sub>Bb<\/jats:sub>\n                      were associated with reduced reactive oxygen species (ROS) because pretreating cells with N\u2010acetyl\u2010L\u2010cysteine and NADPH oxidase inhibitor diphenyleneiodonium (DPI) inhibited LPS\u2010induced TLR4 recruitment into lipid rafts and NF\u2010\u03baB activation. SS\n                      <jats:sub>Bb<\/jats:sub>\n                      also inhibited NADPH oxidase activation by blocking interaction between gp91\n                      <jats:sup>phox<\/jats:sup>\n                      and p47\n                      <jats:sup>phox<\/jats:sup>\n                      similarly as DPI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      SS\n                      <jats:sub>Bb<\/jats:sub>\n                      can inhibit TLR4 recruitment into lipid rafts and its signaling by suppressing the NADPH oxidase\u2010dependent ROS generation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mnfr.201600015","type":"journal-article","created":{"date-parts":[[2016,3,23]],"date-time":"2016-03-23T03:17:33Z","timestamp":1458703053000},"page":"1532-1543","source":"Crossref","is-referenced-by-count":32,"title":["Soyasaponin Bb inhibits the recruitment of toll\u2010like receptor 4 (TLR4) into lipid rafts and its signaling pathway by suppressing the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase\u2010dependent generation of reactive oxygen species"],"prefix":"10.1002","volume":"60","author":[{"given":"Yajie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Nutrition and Food Hygiene Guangdong Provincial Key Laboratory of Tropical Disease Research School of Public Health and Tropical Medicine Southern Medical University  Guangzhou Guangdong P. 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