{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:58:14Z","timestamp":1789430294060,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T00:00:00Z","timestamp":1465171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"publisher","award":["BB\/G007993\/1"],"award-info":[{"award-number":["BB\/G007993\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Early\u2010life exposures are critical in fetal programming and may influence function and health in later life. Adequate maternal folate consumption during pregnancy is essential for healthy fetal development and long\u2010term offspring health. The mechanisms underlying fetal programming are poorly understood, but are likely to involve gene regulation. Epigenetic marks, including DNA methylation, regulate gene expression and are modifiable by folate supply. We observed transcriptional changes in fetal liver in response to maternal folate depletion and hypothesized that these changes are concomitant with altered gene promoter methylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Female C57BL\/6J mice were fed diets containing 2 or 0.4\u2009mg folic acid\/kg for 4\u2009wk before mating and throughout pregnancy. At 17.5\u2010day gestation, genome\u2010wide gene expression and promoter methylation were measured by microarray analysis in male fetal livers. While 989 genes were differentially expressed, 333 promoters had altered methylation (247 hypermethylated, 86 hypomethylated) in response to maternal folate depletion. Only 16 genes had both expression and methylation changes. However, most methylation changes occurred in genomic regions neighboring expression changes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In response to maternal folate depletion, altered expression at the mRNA level was not associated with altered promoter methylation of the same gene in fetal liver.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201600079","type":"journal-article","created":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T05:07:03Z","timestamp":1462165623000},"page":"2031-2042","source":"Crossref","is-referenced-by-count":15,"title":["Gene promoter DNA methylation patterns have a limited role in orchestrating transcriptional changes in the fetal liver in response to maternal folate depletion during pregnancy"],"prefix":"10.1002","volume":"60","author":[{"given":"Jill A.","family":"McKay","sequence":"first","affiliation":[{"name":"Human Nutrition Research Centre Institute of Health and Society, Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiel","family":"Adriaens","sequence":"additional","affiliation":[{"name":"Maastricht Centre for Systems Biology\u2014MaCSBio Maastricht University  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris T.","family":"Evelo","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics\u2014BiGCaT Maastricht University  Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianne","family":"Ford","sequence":"additional","affiliation":[{"name":"Human Nutrition Research Centre Institute for Cell and Molecular Biosciences Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Mathers","sequence":"additional","affiliation":[{"name":"Human Nutrition Research Centre Institute of Cellular Medicine Newcastle University  Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0708473"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyq028"},{"key":"e_1_2_8_4_1","first-page":"Cd007950","article-title":"Effects and safety of periconceptional folate supplementation for preventing birth defects","volume":"10","author":"De\u2010Regil L. 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