{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:26:34Z","timestamp":1788852394637,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T00:00:00Z","timestamp":1486512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31271814"],"award-info":[{"award-number":["31271814"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>The overproduction of very low density lipoprotein (VLDL) is an important cause for initiation and development of atherosclerosis, which is highly associated with insulin signaling. The aim of this work is to verify whether the inhibition of VLDL overproduction is an underlying mechanism for a <jats:italic>Laminaria japonica<\/jats:italic> polysaccharide (LJP61A (where LJP is <jats:italic>L. japonica<\/jats:italic>)) to resist atherosclerosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>LJP61A (50 and 200 mg\/kg\/day) was orally administered to a high\u2010fat diet (HFD)\u2010fed LDL receptor deficient mice for 14 weeks. LJP61A significantly attenuated insulin resistance, hepatic steatosis, atherosclerosis, and dyslipidemia. Meanwhile, LJP61A ameliorated the HFD\u2010induced impairment of hepatic insulin signaling and reduced VLDL overproduction via regulating the expression of genes involved in the assembly and secretion of VLDL. To study the possibility that the inhibition of mammalian target of rapamycin complex 1 and stimulation of Forkhead box protein O1 (Foxo1) nuclear exclusion is a result of LJP61A via regulating insulin signaling, LJP61A was administrated to HepG2 cells in the presence or absence of mTOR inhibitor and Foxo1 inhibitor. Results showed that LJP61A alleviated VLDL overproduction via regulating insulin receptor substrate mediated phosphatidylinositide 3\u2010kinase AKT mammalian target of rapamycin complex 1 and phosphatidylinositide 3\u2010kinase AKT\u2010Foxo1 signaling pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggested that LJP61A ameliorated HFD\u2010induced insulin resistance to attenuate VLDL overproduction possibly via regulating insulin signaling, leading to the inhibition of atherosclerosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201600456","type":"journal-article","created":{"date-parts":[[2016,12,8]],"date-time":"2016-12-08T05:52:52Z","timestamp":1481176372000},"source":"Crossref","is-referenced-by-count":25,"title":["Alleviating VLDL overproduction is an important mechanism for <i>Laminaria japonica<\/i> polysaccharide to inhibit atherosclerosis in <i>LDLr<sup>\u2212\/\u2212<\/sup><\/i> mice with diet\u2010induced insulin resistance"],"prefix":"10.1002","volume":"61","author":[{"given":"Xue\u2010Qiang","family":"Zha","sequence":"first","affiliation":[{"name":"School of Biological and Medical Engineering Hefei University of Technology  Hefei China"},{"name":"School of Food Science and Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010Hua","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Xue","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biological and Medical Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Ping","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Food Science and Engineering Hefei University of Technology  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2538"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201100785"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.160192"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI67227"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.45.3.S24"},{"key":"e_1_2_9_7_1","first-page":"1029","article-title":"Hepatic insulin signaling regulates VLDL secretion and atherogenesis in mice","volume":"119","author":"Han S.","year":"2009","journal-title":"J. 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