{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:12:23Z","timestamp":1791763943366,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,3,21]],"date-time":"2017-03-21T00:00:00Z","timestamp":1490054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000317","name":"Action Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000317","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["097820\/Z\/11\/B"],"award-info":[{"award-number":["097820\/Z\/11\/B"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Nutrition Food Res"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Scope<\/jats:title><jats:p>Low maternal folate status during pregnancy increases the risk of delivering small for gestational age (SGA) infants, but the mechanistic link between maternal folate status, SGA, and placental dysfunction is unknown. microRNAs (miRNAs) are altered in pregnancy pathologies and by folate in other systems. We hypothesized that low maternal folate status causes placental dysfunction, mediated by altered miRNA expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>A prospective observational study recruited pregnant adolescents and assessed third trimester folate status and placental function. miRNA array, QPCR, and bioinformatics identified placental miRNAs and target genes. Low maternal folate status is associated with higher incidence of SGA infants (28% versus 13%, <jats:italic>p<\/jats:italic> &lt; 0.05) and placental dysfunction, including elevated trophoblast proliferation and apoptosis (<jats:italic>p<\/jats:italic> &lt; 0.001), reduced amino acid transport (<jats:italic>p<\/jats:italic> &lt; 0.01), and altered placental hormones (pregnancy\u2010associated plasma protein A, progesterone, and human placental lactogen). miR\u2010222\u20103p, miR\u2010141\u20103p, and miR\u201034b\u20105p were upregulated by low folate status (<jats:italic>p<\/jats:italic> &lt; 0.05). Bioinformatics predicted a gene network regulating cell turnover. Quantitative PCR demonstrated that key genes in this network (zinc finger E\u2010box binding homeobox 2, v\u2010myc myelocytomatosis viral oncogene homolog (avian), and cyclin\u2010dependent kinase 6) were reduced (<jats:italic>p<\/jats:italic> &lt; 0.05) in placentas with low maternal folate status.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study supports that placental dysfunction contributes to impaired fetal growth in women with low folate status and suggests altered placental expression of folate\u2010sensitive miRNAs and target genes as a mechanistic link.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mnfr.201600646","type":"journal-article","created":{"date-parts":[[2017,1,20]],"date-time":"2017-01-20T09:48:46Z","timestamp":1484905726000},"source":"Crossref","is-referenced-by-count":36,"title":["Placental dysfunction is associated with altered microRNA expression in pregnant women with low folate status"],"prefix":"10.1002","volume":"61","author":[{"given":"Bernadette C.","family":"Baker","sequence":"first","affiliation":[{"name":"Maternal and Fetal Health Research Centre University of Manchester  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona L.","family":"Mackie","sequence":"additional","affiliation":[{"name":"Centre of Women's and Newborn's Health &amp; Institute of Metabolism and Systems Research University of Birmingham  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha C.","family":"Lean","sequence":"additional","affiliation":[{"name":"Maternal and Fetal Health Research Centre University of Manchester  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan L.","family":"Greenwood","sequence":"additional","affiliation":[{"name":"Maternal and Fetal Health Research Centre University of Manchester  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander E. 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